Team:Tec-Monterrey/Timeline

From 2010.igem.org

(Difference between revisions)
m
m
Line 305: Line 305:
background-color:#05182b;
background-color:#05182b;
height:15px;
height:15px;
 +
}
 +
.righ .top {
 +
padding:0px 0px 0px 0px;
}
}
.righ .hd .g,
.righ .hd .g,
Line 346: Line 349:
.texteando .bdy {
.texteando .bdy {
padding:0px 15px 0px 15px;
padding:0px 15px 0px 15px;
 +
}
 +
.texteando .top {
 +
padding:0px 0px 0px 0px;
}
}
.texteando .bgr{
.texteando .bgr{
Line 356: Line 362:
  height:13px;
  height:13px;
}
}
-
 
.texteando .ft .c {
.texteando .ft .c {
  height:14px;
  height:14px;
Line 363: Line 368:
  background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;
  background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;
}
}
-
 
.texteando .ft .c {
.texteando .ft .c {
  background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;
  background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;
Line 370: Line 374:
  background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;
  background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;
}
}
-
 
.texteando .hd .g {
.texteando .hd .g {
  background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;
  background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;
 +
}
 +
.bbody2{
 +
margin-left:auto;
 +
margin-right:auto;
 +
width:715px;
 +
}
 +
.smallizq {
 +
float:left;
 +
width:300px;
 +
background-color:#ffffff;
 +
margin:5px 5px 10px 0px;
 +
}
 +
.smallizq .hd .g,
 +
.smallizq .ft .c {
 +
font-size:1px; /* ensure minimum height */
 +
height:13px;
 +
}
 +
.smallizq .bdy {
 +
padding:0px 15px 0px 15px;
 +
}
 +
.smallizq .bgr{
 +
background-color:#05182b;
 +
height:15px;
 +
}
 +
.smallizq .ft .c {
 +
height:14px;
 +
}
 +
.smallizq .ft {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;
 +
}
 +
.smallizq .ft .c {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;
 +
}
 +
.smallizq .hd {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;
 +
}
 +
.smallizq .hd .g {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;
 +
}
 +
.smallizq .top {
 +
padding:0px 0px 0px 0px;
 +
}
 +
 +
 +
 +
 +
 +
.smallder {
 +
float:right;
 +
width:300px;
 +
background-color:#ffffff;
 +
margin:5px 5px 10px 0px;
 +
}
 +
.smallder .hd .g,
 +
.smallder .ft .c {
 +
font-size:1px; /* ensure minimum height */
 +
height:13px;
 +
}
 +
.smallder .bdy {
 +
padding:0px 15px 0px 15px;
 +
}
 +
.smallder .bgr{
 +
background-color:#05182b;
 +
height:15px;
 +
}
 +
.smallder .ft .c {
 +
height:14px;
 +
}
 +
.smallder .ft {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/8/85/Bl.png) no-repeat 0px 0px;
 +
}
 +
.smallder .ft .c {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/b/bf/Br.png) no-repeat right 0px;
 +
}
 +
.smallder .hd {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/9/9b/Tl.png) no-repeat 0px 0px;
 +
}
 +
.smallder .hd .g {
 +
background:transparent url(https://static.igem.org/mediawiki/2010/5/5f/Tr.png) no-repeat right 0px;
 +
}
 +
.smallder .top {
 +
padding:0px 0px 0px 0px;
}
}
Line 621: Line 706:
<div class="righ">
<div class="righ">
<div class="hd"><div class="g"></div></div>
<div class="hd"><div class="g"></div></div>
 +
<div class="top"><img src="https://static.igem.org/mediawiki/2010/7/7e/Sponsorstec.png">
 +
</div>
<div class="bdy">
<div class="bdy">
-
<h2>  Sponsors:</h2>
 
-
 
<p align="center">
<p align="center">
<a href="http://www.uniparts.com.mx/"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a>
<a href="http://www.uniparts.com.mx/"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a>
Line 641: Line 726:
<div class="bgr"></div>
<div class="bgr"></div>
<div class="hd"><div class="g"></div></div>
<div class="hd"><div class="g"></div></div>
 +
<div class="top"><img src="https://static.igem.org/mediawiki/2010/4/4b/Followuson.png">
 +
</div>
<div class="bdy">
<div class="bdy">
-
<h2>Follow us on:</h2>
 
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a>
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a>
</div>
</div>
Line 651: Line 737:
<div class="texteando">
<div class="texteando">
<div class="hd"><div class="g"></div></div>
<div class="hd"><div class="g"></div></div>
 +
<div class="top">
 +
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" >
<div class="bdy">
<div class="bdy">
<div style="text-align:justify">
<div style="text-align:justify">
-
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" >
 
<p class="blah">
<p class="blah">
       Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a>
       Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a>
Line 681: Line 768:
</div>
</div>
<div class="hd"><div class="g"></div></div>
<div class="hd"><div class="g"></div></div>
 +
<div class="top">
 +
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" >
<div class="bdy">
<div class="bdy">
<h1>Project Summary</h1>
<h1>Project Summary</h1>
Line 706: Line 795:
</div>
</div>
<div class="ft"><div class="c"></div></div>
<div class="ft"><div class="c"></div></div>
 +
<div class="bgr"><br>
 +
</div>
 +
</div>
-
 
+
<div class="ft"> </div>
-
 
+
</div>
</div>
 +
</div> /* Este DIV es el de TEXTEANDO */
 +
</div>
 +
<div class="bgr"><br>
 +
</div>
 +
<div class="bbody2">
 +
<div class="smallizq">
 +
<div class="hd"><div class="g"></div></div>
 +
<div class="top">
 +
<img src="https://static.igem.org/mediawiki/2010/4/42/Welcome.png" border="0" >
 +
</div>
 +
<div class="bdy">
 +
<div style="text-align:justify">
 +
<p class="blah">
 +
      Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of
 +
</div>
 +
</div>
 +
<div class="ft"><div class="c"></div></div>
 +
 +
<div class="smallder">
 +
Blablablablabalb
 +
</div>
</div>
</div>
</html>
</html>

Revision as of 05:22, 5 October 2010

Tec de Monterrey




























Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology (van der Meer and Belkin, 2010). There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes (Yagi, 2007)


The first bacterial reporters appeared 20 years ago (van der Meer and Belkin, 2010) although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants (Willardson, et. Al., 1998) and sugar and amino acid availability in soils (Jaeger, et. Al., 1999). Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration.


We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” (van der Meer and Belkin, 2010) capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings.


With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.

References

Jaeger, C. H., et. Al. (June 1999) Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690

Van der Meer, J. R. and Belkin, S. (July 2010) Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522

Willardson, B. M., et. Al. (March 1998) Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012

Yagi, K. (2007) Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258


Project Summary

Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology (van der Meer and Belkin, 2010). There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes (Yagi, 2007)


The first bacterial reporters appeared 20 years ago (van der Meer and Belkin, 2010) although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants (Willardson, et. Al., 1998) and sugar and amino acid availability in soils (Jaeger, et. Al., 1999). Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration.


We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” (van der Meer and Belkin, 2010) capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings.


With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.

References

Jaeger, C. H., et. Al. (June 1999) Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690

Van der Meer, J. R. and Belkin, S. (July 2010) Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522

Willardson, B. M., et. Al. (March 1998) Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012

Yagi, K. (2007) Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258


/* Este DIV es el de TEXTEANDO */

Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology (van der Meer and Belkin, 2010). There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of

Blablablablabalb