Team:Peking

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<p><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<font size=4>Biosensor</font><br>
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<p><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<font size=4>Biosensor:</font><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Based on reverse engineering principles, we<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Based on reverse engineering principles, we<br>
&nbsp;&nbsp;&nbsp;engineered our bacteria into valid bioreporters <br>&nbsp;&nbsp;&nbsp;for Hg(II) in aquatic environment. Rational desi-<br>&nbsp;&nbsp;&nbsp;-gn of genetic circuit topology was conducted to <br>&nbsp;&nbsp;&nbsp;confer mercury sensor and regulator compone-<br>&nbsp;&nbsp;&nbsp;-nts high efficiency and robustness. Additionally,<br>&nbsp;&nbsp;&nbsp; a new approach based on the use of multiple <br>&nbsp;&nbsp;&nbsp;bioreporter cell lines to control assay variations <br>&nbsp;&nbsp;&nbsp;and to improve in field application ease was also <br>&nbsp;&nbsp;&nbsp;developed. <br>
&nbsp;&nbsp;&nbsp;engineered our bacteria into valid bioreporters <br>&nbsp;&nbsp;&nbsp;for Hg(II) in aquatic environment. Rational desi-<br>&nbsp;&nbsp;&nbsp;-gn of genetic circuit topology was conducted to <br>&nbsp;&nbsp;&nbsp;confer mercury sensor and regulator compone-<br>&nbsp;&nbsp;&nbsp;-nts high efficiency and robustness. Additionally,<br>&nbsp;&nbsp;&nbsp; a new approach based on the use of multiple <br>&nbsp;&nbsp;&nbsp;bioreporter cell lines to control assay variations <br>&nbsp;&nbsp;&nbsp;and to improve in field application ease was also <br>&nbsp;&nbsp;&nbsp;developed. <br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Project/Biosensor"><font size=4>---learn more</font></a></p>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Project/Biosensor"><font size=4>---learn more</font></a></p>
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<p><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<font size=4>Expansion:</font>
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&nbsp;&nbsp;&nbsp;We noticed that MerR family TFs share a high ho-<br>&nbsp;&nbsp;&nbsp;-mology at their metal binding domains, which implies that our strategies of &nbsp;&nbsp;&nbsp;bioreporter and bioabsorbent engineering might be applicable to other cases. <br>&nbsp;&nbsp;&nbsp;Then our reverse engineering strategy was expanded to another common to-<br>&nbsp;&nbsp;&nbsp;-xic heavy metal, lead (Pb) to prove the validness of our engineering strategy.
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Revision as of 14:05, 7 October 2010

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       Biosensor:
       Based on reverse engineering principles, we
   engineered our bacteria into valid bioreporters
   for Hg(II) in aquatic environment. Rational desi-
   -gn of genetic circuit topology was conducted to
   confer mercury sensor and regulator compone-
   -nts high efficiency and robustness. Additionally,
    a new approach based on the use of multiple
   bioreporter cell lines to control assay variations
   and to improve in field application ease was also
   developed.
                                           ---learn more


       Expansion:    We noticed that MerR family TFs share a high ho-
   -mology at their metal binding domains, which implies that our strategies of    bioreporter and bioabsorbent engineering might be applicable to other cases.
   Then our reverse engineering strategy was expanded to another common to-
   -xic heavy metal, lead (Pb) to prove the validness of our engineering strategy.

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