Team:Peking

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    <h1><b>Project Description</b></h1>
 
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<p><b>In the field of biodetection and bioremediation, despite numerous proofs of principle, most bioreporters and bioactuators have remained confined to the laboratory. Also bacterial reporters and actuators have NOT been rational enough in design and NOT complex enough in function.Additionally, we notice that genetic manipulation in this field needs streamline methods and it's time for a series of issues on the field application to be taken into consideration. </b></p>
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<p><b>In this summer, we are engineering our bacteria to resolve these hard truths mentioned above. Firstly, we exploit MerR family transcription factors and rational designs such as dose-response alignment to construct a series of biorepoters for heavy metal detection. Rational design for genetic circuits enables cellular sensory and regulatory components to work much more efficiently and robustly. These bioreporters are expected to discriminate concentration ranges of certain heavy metal in water independently of incubation time and bioreporter activity. This means that the field application can be carried out without the need for costly equipment while response quality and sensor sensitivity are still kept. Secondly, we are constructing bioactuator strains that decontaminate heavy metals from polluted water. This will be accomplished by inductively expressing engineered MerR family TFs on surface, periplasm and cytosol of E.coli cells. In our bioactuators, MerR family TFs are engineered into single-chain, antiparallel, coiled coil peptides that mimic the metal binding domains, which will reduce costs of metal binding. As MerR family TFs share high homology and almost each species of heavy metal has a corresponding MerR family TF, we can say we are developing a streamlined method to construct heavy metal decontamination kits which are composed of high-performance bioreporters and bioactuators.</b></p>
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<a class="B1" a href="https://2010.igem.org/Team:Peking/Project/Biosensor">Biosensor<img src="https://static.igem.org/mediawiki/2010/1/1b/Sensorintro.png"/></a>
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<a class="B2" a href="https://2010.igem.org/Team:Peking/Project/Bioabsorbent">Bioabsorbent<img src="https://static.igem.org/mediawiki/2010/8/84/Absorbentintro.png"/></a>
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<a class="B3" a href="https://2010.igem.org/Team:Peking/Project/Application">Application<img src="https://static.igem.org/mediawiki/2010/b/b6/Applicationintro.png"/></a>
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<a class="B4" a href="https://2010.igem.org/Team:Peking/Project/Expansion">Expansion<img src="https://static.igem.org/mediawiki/2010/b/b1/Expansionintro.png"/></a>
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==Parts==
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<html><a href="https://2010.igem.org/Team:Peking/Parts"><img src="https://static.igem.org/mediawiki/2010/d/da/Pku_part3.jpg" width=350 height=135></a></html>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <html><a href="https://2010.igem.org/Team:Peking/Parts"><font size=4>learn more</font></a></p></html>
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    <h2>*****</h2>
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==Team==
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<html><a href="https://2010.igem.org/Team:Peking/Team"><font size=4>learn more</font></a></p></html>
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==Modeling==
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<html><a href="https://2010.igem.org/Team:Peking/Modeling"><img src="https://static.igem.org/mediawiki/2010/7/76/Modelingpku.jpg" width=500 height=230 alt="Modeling"  ></a>
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&nbsp;&nbsp;&nbsp;&nbsp;We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Modeling"><font size=4>learn more</font></a>
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==Human Practice==
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<html><a href="https://2010.igem.org/Team:Peking/Humanpractice"><img src="https://static.igem.org/mediawiki/2010/8/84/FrontpageHP.jpg" width=350 height=127 alt="Biosafety"  align="right"></a>
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&nbsp;&nbsp;&nbsp;&nbsp;DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner. <br>
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&nbsp;&nbsp;&nbsp;&nbsp; This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2010.igem.org/Team:Peking/Humanpractice"><font size=4>learn more</font></a>
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==Gallery==
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<p>&nbsp;&nbsp;&nbsp;&nbsp;It's an unforgettable summer, during which we spared no effort to fight for our dreams and the honour of Peking iGEM team. Photos recorded every footprint of everyone. Join us together!</p>
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<img src="https://static.igem.org/mediawiki/2010/2/2d/PKU_IGEM20102.gif" width="100px" alt="the whole team" >&nbsp;
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&nbsp;<img src="https://static.igem.org/mediawiki/2010/2/2f/%E5%9B%BE%E5%83%8F0201.jpg" width="100px" alt="Balloon on the experiment table" width="50px" >&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/0/03/Rats.gif" width="100px" alt="Team's mascot~" >&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/4/46/Assembled.jpg" width="100px" alt="Our lab">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/8/88/P_large_oReI_3ff9000598fc2d11.jpg" width="115px" alt="Ao Liu & Ying Shen's birthday party">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/1/1c/P_large_MA7H_33dd00001e802d0b.jpg" width="230px" alt="Weekly Meeting"></a> &nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/a/ab/Pkupuzzle521.jpg" width="100px" alt="Team's pyjama~"></a></html>
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<font size=5>Count Down</font><br><br>
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Latest revision as of 22:17, 27 October 2010

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Parts

    Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.

                                                          learn more

Team

    Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.


                                                                                                 learn more

Modeling

Modeling     We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.
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Human Practice

Biosafety     DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner.
     This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.
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