Team:METU Turkey/Results Discussion/IPTG induction




In expression comparison with IPTG concentration experiment, we realized that our CooA pTriEx vector is expressed without IPTG induction however when the cultures were induced with IPTG the expression incresed a little. Thus, we concluded that IPTG concentration doesn't much affect our expression yield. However, when 2mM IPTG was added to culture,expression of CooA was decreased. It has probably a toxic affect on our bacteria.