Team:METU Turkey/Results Discussion

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1-Construction of Cell Sensor modules

Design and ordering of DNA parts - Ordering of parts from GENEART: We have ordered 11 gene sequences from GENEART. We have synthesized pCooM, pCooF promoter and its 4 variants, CooA protein and 4 of its variants. Those genes are proven to be successful in cloning. - Design of parts: We have designed two different vectors , one containing pTriEx vector containing CooA and one containing our final construct. However, we couldn’t observe fluorescence with this system because of high expression capability of pTriEx. Because of this factor, we have designed another biobrick containing pLacI-RBS-RFP. We have cloned this with pCooM-RBS-RFP-TT and pSB1C3. We didn’t have enough time for cloning of the final vector with this biobrick. .

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-<h2>Gelatin Sponge</h2>
+<h2>1-Construction of Cell Sensor modules</h2>
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-<html><div align="center" style="padding-left: 66px; padding-top: 8px;"><img style="border: 0px solid ; width: 250px; height: 250px;" alt="w6" src="https://static.igem.org/mediawiki/2009/0/01/Ttttgltn.jpg"></a></div></html>
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-<p style="font-size:110%; color:#576f91; font-family:georgia,serif;"><br>It is believed that, epidermal growth factor (EGF) stimulates the growth of keratinocytes in vivo, and therefore plays an important role in the process of wound healing that depends on mitosis and migration of keratinocytes. Rhinewald and Green showed, in vitro that in the presence of growth factors, higher percentage of cells leave the resting state, enter and remain in the mitotic cycle. Assuming a similar effect of EGF on epidermal cells in vivo, the primary mechanism of enhanced wound healing is most likely due to increased proliferation of epidermal cells.</p>
+<p style="font-size:110%; color:#576f91; font-family:georgia,serif;"><br>Design and ordering of DNA parts
+-	Ordering of parts from GENEART:  We have ordered 11 gene sequences from GENEART. We have synthesized pCooM, pCooF promoter and its 4 variants, CooA protein and 4 of its variants. Those genes are proven to be successful in cloning.
+-	Design of parts: We have designed two different vectors , one containing pTriEx vector containing CooA and one containing our final construct. However, we couldn’t observe fluorescence with this system because of high expression capability of pTriEx. Because of this factor, we have designed another biobrick containing pLacI-RBS-RFP. We have cloned this with pCooM-RBS-RFP-TT and pSB1C3. We didn’t have enough time for cloning of the final vector with this biobrick.
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