Team:METU Turkey/Planning

From 2010.igem.org

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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 950px; height: 300px;" alt="w7" src="http://partsregistry.org/wiki/images/d/d0/Schedule.JPG"></a></div>
<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 950px; height: 300px;" alt="w7" src="http://partsregistry.org/wiki/images/d/d0/Schedule.JPG"></a></div>
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<br><h2>PROJECT EXECUTION</h2>
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<br><h2>PLANNING</h2>
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<br>Design and Synthesis of Response Elements
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<br><h3>Cloning</h3>
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<br> - Construction of Cell Sensor modules
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<br> - Preparation of competent cell
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<br> - Design and ordering of DNA parts
<br> - Design and ordering of DNA parts
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<br> - Preparation of competent cells
<br> - Cloning
<br> - Cloning
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<br> - Construction of Cell Sensor modules
<br> - Validation
<br> - Validation
<br> - Assembly of final construct
<br> - Assembly of final construct
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<br>Cell Sensor Experiments
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<br><h3>Cell Sensor Experiments</h3>
<br> - Fermentor studies
<br> - Fermentor studies
<br> - Flask experiments
<br> - Flask experiments
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<br>CooA Expression and Purification
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<br><h3>CooA Expression and Purification</h3>
<br> - Overexpression optimization
<br> - Overexpression optimization
<br> - Purification optimization
<br> - Purification optimization
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<br>Characterization: Isothermal Titration Calorimetry (ITC)
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<br><h3>Characterization: Isothermal Titration Calorimetry (ITC)</h3>
<br> - DNA component preparation
<br> - DNA component preparation
<br> - Optimization studies
<br> - Optimization studies
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<br>Characterization: Electrophoretic Mobility Shift Assay (EMSA)
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<br><h3>Characterization: Electrophoretic Mobility Shift Assay (EMSA)</h3>
<br> - Optimization studies
<br> - Optimization studies
<br> - Determination of Response Element - Transcription Factor Affinities
<br> - Determination of Response Element - Transcription Factor Affinities
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<br>Characterization: Intrinsic Tryptophan Fluorescence (ITF)
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<br><h3>Characterization: Intrinsic Tryptophan Fluorescence (ITF)</h3>
<br> - Optimization studies
<br> - Optimization studies
<br> - Determination of Response Element - Transcription Factor Affinities
<br> - Determination of Response Element - Transcription Factor Affinities
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Latest revision as of 03:33, 28 October 2010

Home

 




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w7


PLANNING




Cloning


- Design and ordering of DNA parts
- Preparation of competent cells
- Cloning
- Construction of Cell Sensor modules
- Validation
- Assembly of final construct

Cell Sensor Experiments


- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy

CooA Expression and Purification


- Overexpression optimization
- Purification optimization

Characterization: Isothermal Titration Calorimetry (ITC)


- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Electrophoretic Mobility Shift Assay (EMSA)


- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Intrinsic Tryptophan Fluorescence (ITF)


- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

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