Team:Lethbridge/Notebook/Lab Work/September
From 2010.igem.org
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<b>Follow-up:</b> TBD<br> | <b>Follow-up:</b> TBD<br> | ||
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- | + | <b>Objective:</b> Create glycerol stocks of J04450 in pSB1A3 and pSB1T3 for use in RFP-BioBrick Assembly.<br> | |
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- | <b>Objective:</b> Create glycerol stocks of J04450 in pSB1A3 | + | |
<b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | <b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | ||
Obtained all plasmid DNA from 2010 Kit Plate 1 | Obtained all plasmid DNA from 2010 Kit Plate 1 | ||
*J04450 in pSB1A3 - Well 1C | *J04450 in pSB1A3 - Well 1C | ||
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*J04450 in pSB1T3 - Well 7A | *J04450 in pSB1T3 - Well 7A | ||
<b>Results:</b> Obtained TNTC colonies<br> | <b>Results:</b> Obtained TNTC colonies<br> | ||
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*5) Submit to registry for sequencing | *5) Submit to registry for sequencing | ||
==<font color="white">September 24, 2010== | ==<font color="white">September 24, 2010== | ||
- | ===ADS | + | ===ADS=== |
+ | <b>Objective:</b> Generate plasmid DNA of E1010 for downstream PCR<br> | ||
+ | <b>Method:</b> Transform plasmid DNA into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | ||
+ | DNA obtained from 2010 Kit Plate 1 Well 18F (E1010 in pSB2K3)<br> | ||
+ | <b>Results:</b> Obtained <font color="red"> TBD</font> colonies<br> | ||
+ | <b>Follow-up:</b> | ||
+ | *Grow overnight cultures (Generate glycerol stocks) | ||
+ | *Purify plasmid DNA (Generate pDNA stocks) | ||
+ | *PCR to add terminal fusion standards | ||
+ | ---------------------------------------------------------------------------------------------------------------------- | ||
+ | <b>Objective:</b> Create glycerol stocks of J04450 in pSB1K3 for use in RFP-BioBrick Assembly.<br> | ||
+ | <b>Method:</b> Transform into Subcloning Efficiency Competent DH5α Cells (Invitrogen)<br> | ||
+ | Obtained plasmid DNA from 2010 Kit Plate 1 well 5A (J04450 in pSB1K3) | ||
+ | <b>Results:</b> Obtained TNTC colonies<br> | ||
+ | <b>Follow-up:</b> | ||
+ | *Grow overnight cultures | ||
+ | *Generate Glycerol Stocks | ||
+ | *Generate Plasmid DNA via Maxiprep |