Team:Kyoto/Materials

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Contents

Materials

Strains

NameGenotype and/or Relevant FeaturesSource or Reference
C2
TOP10F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ-Invitrogen
KRX[F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]
BL21(DE3)pLysSF–, ompT, hsdSB (rB, mB), dcm, gal, λ(DE3), pLysS, Cmr.Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]

DNA

NameSource or Reference
Lambda Phage (cI857 Sam7)DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399]

Primers

NameSequence

Reagent

NameSupplier
Ampicillin
Kanamycin
Chloramphenicol
EcoRI
XbaI
SpeI
PstI
DpnI
Isopropyl β-D-1-Thiogalactopyranoside (IPTG)
Ligation High