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Team:Kyoto/Materials
From 2010.igem.org
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|TOP10||F-, ''mcr''A, Δ(''mrr''-''hsd''RMS-''mcr''BC), φ80''lac''ZΔM15, Δ''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, Δ(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, λ-||Invitrogen | |TOP10||F-, ''mcr''A, Δ(''mrr''-''hsd''RMS-''mcr''BC), φ80''lac''ZΔM15, Δ''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, Δ(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, λ-||Invitrogen | ||
|- | |- | ||
| - | | | + | |rowspan="2"|KRX||rowspan="2"|[F′, ''tra''D36, Δ''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, Δ(''lac''Z)M15] Δ''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, Δ(''lac''-''pro''AB), Δ(''rha''BAD)::T7 RNA polymerase.||Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University |
|- | |- | ||
|(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]) | |(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]) | ||
Revision as of 13:55, 23 October 2010
LastModified: 2010-10-23
Contents |
Materials
Strains
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ- | Invitrogen |
| KRX | [F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase. | Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University |
| (Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]) | ||
| BL21(DE3)pLysS | F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] |
DNA
| Name | Source or Reference |
|---|---|
| Lambda Phage (cI857 Sam7) | DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] |
| http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 |
Primers
| Date | Name | Sequence |
|---|---|---|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt |
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc |
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc |
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg |
| 08/23, 08/24 | Deletion for Upstream Forward | gagcaaatccccttattgggggtaagacatg |
| 08/23, 08/24 | Deletion for Upstream Reverse | ctctagaagcggccgcgaattcc |
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc |
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc |
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa |
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc |
