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Team:Kyoto/Materials
From 2010.igem.org
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|BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', λ(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] | |BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', λ(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] | ||
| + | |} | ||
| + | |||
| + | ===DNA=== | ||
| + | {| class="materials" | ||
| + | !Name||Source or Reference | ||
| + | |- | ||
| + | |Lambda Phage (cI857 Sam7)||DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] | ||
| + | |} | ||
| + | |||
| + | ===Primers=== | ||
| + | {| class="materials" | ||
| + | !Name||Sequence|| | ||
|} | |} | ||
Revision as of 18:11, 20 October 2010
LastModified: 2010-10-20
Contents |
Materials
Strains
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| C2 | ||
| TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ- | Invitrogen |
| KRX | [F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698] |
| BL21(DE3)pLysS | F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] |
DNA
| Name | Source or Reference |
|---|---|
| Lambda Phage (cI857 Sam7) | DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] |
Primers
| Name | Sequence |
|---|
Reagent
| Name | Supplier |
|---|---|
| Ampicillin | |
| Kanamycin | |
| Chloramphenicol | |
| EcoRI | |
| XbaI | |
| SpeI | |
| PstI | |
| DpnI | |
| Isopropyl β-D-1-Thiogalactopyranoside (IPTG) | |
| Ligation High |
