Team:HokkaidoU Japan/NotebookTest

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Calendar

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Abstract

Monday, July 12, 2010

  • The first experiment for us, transformation with BioBrick Devices.

Wednesday, July 21, 2010

  • Preparation of competent cells
  • Single colony isolation of E. coli which had been transformed on Monday, July 12, 2010

Monday, July 26, 2010

  • Cultivation of transformed E. coli for the next day's miniprep

Tuesday, July 27, 2010

  • Miniprep

Monday, August 2, 2010

  • Restriction enzyme digestion of plasmids which had been purified by miniprep
  • Electrophoresis assay

Tuesday, August 3, 2010

Wednesday, August 4, 2010

Thursday, August 5, 2010

Friday, August 6, 2010


Monday, August 9, 2010

  • Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive and heat-sensitive E. coli
  • This is a preliminary experiment before starting the main project

Tuesday, August 10, 2010

  • PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis for confirmation

Wednesday, August 11, 2010

  • Preparation for the next day's miniprep
  • Estimation of enzyme activity
  • Preparation of the glycerol stocks

Thursday, August 12, 2010

  • Miniprep (1-18F, 2-21H and 2-11P)
  • Electrophoresis of the DNA which had amplified via PCR and miniprep

Friday, August 13, 2010

  • PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)

Monday, August 16, 2010

  • Measurement of restriction enzyme activity
  • Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)

Tuesday, August 17, 2010

  • Purification of the DNA solutions via gel extraction
  • Preparation of agar medium containing 35 ug/mL chloramphenicol

Wednesday, August 18, 2010

Thursday, August 19, 2010

Friday, August 20, 2010


Monday, August 23, 2010

Tuesday, August 24, 2010

Wednesday, August 25, 2010

Thursday, August 26, 2010

Friday, August 27, 2010


Monday, August 30, 2010

Tuesday, August 31, 2010

Wednesday, September 1, 2010

Thursday, September 2, 2010

Friday, September 3, 2010


Monday, September 6, 2010

Tuesday, September 7, 2010

Wednesday, September 8, 2010

Thursday, September 9, 2010

Friday, September 10, 2010


Monday, September 13, 2010

Tuesday, September 14, 2010

Wednesday, September 15, 2010

Thursday, September 16, 2010

Friday, September 17, 2010


Monday, September 20, 2010

Tuesday, September 21, 2010

Wednesday, September 22, 2010

Thursday, September 23, 2010

Friday, September 24, 2010


Monday, September 27, 2010

Tuesday, September 28, 2010

Wednesday, September 29, 2010

Thursday, September 30, 2010

Friday, October 1, 2010


Monday, October 4, 2010

Tuesday, October 5, 2010

Wednesday, October 6, 2010

Thursday, October 7, 2010

Friday, October 8, 2010


Monday, October 11, 2010

Tuesday, October 12, 2010

Wednesday, October 13, 2010

Thursday, October 14, 2010

Friday, October 15, 2010


Monday, October 18, 2010

Tuesday, October 19, 2010

Wednesday, October 20, 2010

Thursday, October 21, 2010

Friday, October 22, 2010


Monday, October 25, 2010

Tuesday, October 26, 2010

Wednesday, October 27, 2010

Thursday, October 28, 2010

Friday, October 29, 2010


Monday, November 1, 2010

Tuesday, November 2, 2010

Wednesday, November 3, 2010

Thursday, November 4, 2010

Friday, November 5, 2010


Monday, November 8, 2010

Tuesday, November 9, 2010

Wednesday, November 10, 2010

Thursday, November 11, 2010

Friday, November 12, 2010


Monday, November 15, 2010

Tuesday, November 16, 2010

Wednesday, November 17, 2010

Thursday, November 18, 2010

Friday, November 19, 2010


Monday, November 22, 2010

Tuesday, November 23, 2010

Wednesday, November 24, 2010

Thursday, November 25, 2010

Friday, November 26, 2010


Monday, November 29, 2010

===Tuesday, November 30, 2010===