Team:Debrecen-Hungary/protocols
From 2010.igem.org
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=== Ligand Treatment === | === Ligand Treatment === | ||
[http://openwetware.org/wiki/IGEM:University_of_Debrecen:Ligand_Treatment ''Main article - Ligand treatment''] <br> | [http://openwetware.org/wiki/IGEM:University_of_Debrecen:Ligand_Treatment ''Main article - Ligand treatment''] <br> | ||
- | Ligand treatment is a procedure when we add the appropriate ligand to the nuclear receptor (NR, transcription factor). The NR got into the cells through a previous transfection step. After treatment the ligand-binded NRs will dimerize and bind to the DNA at specific Nuclear receptor Response Elements and this will promote the gene expression of the downstream gene. In further examinations we detect the expression level of the target gene by Luciferase assay. [ | + | Ligand treatment is a procedure when we add the appropriate ligand to the nuclear receptor (NR, transcription factor). The NR got into the cells through a previous transfection step. After treatment the ligand-binded NRs will dimerize and bind to the DNA at specific Nuclear receptor Response Elements and this will promote the gene expression of the downstream gene. In further examinations we detect the expression level of the target gene by Luciferase assay. [https://2010.igem.org/Team:Debrecen-Hungary/protocols/Ligand_Treatment Read more...] |
[[Team:Debrecen-Hungary/protocols#Notebook|[TOP]]] | [[Team:Debrecen-Hungary/protocols#Notebook|[TOP]]] | ||
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=== Measuring Luciferase activity with the Victor plate reader === | === Measuring Luciferase activity with the Victor plate reader === | ||
[http://openwetware.org/wiki/IGEM:University_of_Debrecen:_Enzymatic_activity_with_Victor_machine ''Main article - easuring Luciferase activity with the Victor plate reader''] <br> | [http://openwetware.org/wiki/IGEM:University_of_Debrecen:_Enzymatic_activity_with_Victor_machine ''Main article - easuring Luciferase activity with the Victor plate reader''] <br> | ||
- | he Wallac 1420 VICTOR2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. An extended version of the successful Wallac VICTOR multilabel reader, the VICTOR2 allows immediate access to more than 10 counting modes, covering all of the main nonradioactive counting technologies. [ | + | he Wallac 1420 VICTOR2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. An extended version of the successful Wallac VICTOR multilabel reader, the VICTOR2 allows immediate access to more than 10 counting modes, covering all of the main nonradioactive counting technologies. [https://2010.igem.org/Team:Debrecen-Hungary/protocols/_Enzymatic_activity_with_Victor_machine Read more...] |
[[Team:Debrecen-Hungary/protocols#Notebook|[TOP]]] | [[Team:Debrecen-Hungary/protocols#Notebook|[TOP]]] |
Revision as of 14:56, 4 October 2010
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