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Team:Chiba/System 1/Result
From 2010.igem.org
Version 1 :
Requirement of realizing genetic double click system
1. T7 RNAP pulse in response to 1st and 2nd input
In response to 1st and 2nd input, T7 RNAP has to express as pulse.
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.
So, we checked this part.
2. CI repression
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to
have degradated before 2nd input not to work as a repressor at 2nd input.
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.
So, we checked this part).
3. Setting the fixed time
There is the fixed time between 1st and 2nd input.
To accomplish this, CI has to re-express after washout. (Data not shown)
1-2. Pulse generator
