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| | </body> | | </body> |
| | <!--- 2nd tab End---> | | <!--- 2nd tab End---> |
| - | <!--- 3rd tab--->
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| - | <div id="search-controls">
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| - | <form action="/Special:Search" id="searchform">
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| - | <input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" />
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| - | <input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />
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| - | <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
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| - | </form>
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| - | </div> <!-- close search controls -->
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| - | <p>
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| - | <style type="text/css">
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| - | <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
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| - | <html xmlns="http://www.w3.org/1999/xhtml">
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| - | <head>
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| - | <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
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| - | <title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title>
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| - | <style type="text/css">
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| - | <!--
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| - | body {
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| - | margin:0;
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| - | padding:0;
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| - | font: bold 11px/1.5em Verdana;
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| - | }
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| - | h2 {
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| - | font: bold 14px Verdana, Arial, Helvetica, sans-serif;
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| - | color: #000;
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| - | margin: 0px;
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| - | padding: 0px 0px 0px 15px;
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| - | }
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| - |
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| - | img {
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| - | border: none;
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| - | }
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| - |
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| - | /*- Menu Tabs 8--------------------------- */
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| - |
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| - | #tabs8 {
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| - | float:left;
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| - | width:100%;
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| - | font-size:100%;
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| - | line-height:normal;
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| - | background:#007500;
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| - | margin-top:0px;
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| - | }
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| - | #tabs8 ul {
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| - | margin:0;
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| - | padding:2px 10px 0 105px;
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| - | list-style:none;
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| - | }
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| - | #tabs8 li {
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| - | float:left;
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| - | display:inline;
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| - | margin:0;
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| - | padding:0;
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| - | margin-left:40px;
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| - | }
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| - | #tabs8 a {
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| - | float:left;
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| - | background:url("tableft10.gif") no-repeat left top;
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| - | margin:0;
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| - | padding:0 0 0 2px;
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| - | text-decoration:none;
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| - | }
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| - | #tabs8 a span {
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| - | float:left;
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| - | display:block;
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| - | background:url("tabright10.gif") no-repeat right top;
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| - | padding:5px 15px 4px 6px;
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| - | color:#FFF;
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| - | }
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| - | /* Commented Backslash Hack hides rule from IE5-Mac \*/
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| - | #tabs8 a span {float:none;}
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| - | /* End IE5-Mac hack */
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| - | #tabs8 a:hover span {
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| - | color:#FFFFFF;
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| - | }
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| - | #tabs8 a:hover {
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| - | background-position:0% -42px;
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| - | }
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| - | #tabs8 a:hover span {
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| - | background-position:100% -42px;
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| - | }
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| - |
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| - | #tabs8 #current a {
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| - | background-position:0% -42px;
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| - | }
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| - | #tabs8 #current a span {
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| - | background-position:100% -42px;
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| - | }
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| - | -->
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| - | </style>
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| - | </head>
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| - |
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| - | <body>
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| - | <div id="tabs8">
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| - | <ul>
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| - | <!-- CSS Tabs -->
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| - | <li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li>
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| - | <li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li>
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| - | <li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li>
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| - | <li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li>
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| - | <li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li>
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| - | </ul>
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| - | </div>
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| - | </body>
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| | </html> | | </html> |
| - | <!--- 3rd tab End --->
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| | <!-- ------------------------------------------------------------------------------- | | <!-- ------------------------------------------------------------------------------- |
| | ------------------------------------------------------------------------------- | | ------------------------------------------------------------------------------- |
1. T7 RNAP pulse in response to 1st and 2nd input
In response to 1st and 2nd input, T7 RNAP has to express as pulse.
To accomplish this, Lux/CI434 hybrid promoter should be activated or repressed stringently.
So, we checked this part.
2. CI repression
CI needs to work as a repressor of T7/CI-OR1 hybrid promoter at 1st input and needs to
have degradated before 2nd input not to work as a repressor at 2nd input.
To accomplish this, T7/CI-OR1 hybrid promoter should be activated or repressed stringently.
So, we checked this part).
3. Setting the fixed time
There is the fixed time between 1st and 2nd input.
To accomplish this, CI has to re-express after washout. (Data not shown)
"
