Team:Cambridge/Tools/Gibson

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Revision as of 14:46, 23 October 2010

Gibson Assembly

Gibthon Construct Designer

is a software tool under active development to aid with the design of primers for Gibson Assembly. It currently comes in two flavours - Gibthon Beta, and Gibthon Construct Designer Beta.

Gibthon Beta - here

The more basic of the two tools, this will take as an input the sequences surrounding the join for which you wish to design primers. You can interactively adjust the length of the primers to tune the melting temperature of the DNA and check for secondary structures.

Gibthon Construct Designer Beta - Here

Gibthon's older brother, and much more powerful. With this, you can design your construct from its component genes and automatically generate the optimal primers. The tool has access to the Parts Registry Database, the NCBI's Nucleotide database, as well as accepting genbank formatted files from the user.

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 {{:Team:Cambridge/Templates/headerMinimalprototype}}
 {{:Team:Cambridge/Templates/headerbar|colour=#386abc|title=Gibson Assembly}}
-<html><div style="float:left; padding:0px 10px;"><a href="http://www.gibthon.org"><img src="http://www.gibthon.org/images/gibthon.png" /></a></div><div style="height:300px;"><a href="www.gibthon.org"><h3>Gibthon</h3></a> is a tool written in python to aid with the design of primers for Gibson Assembly. It's out in a very early version, so any testing you could do on it would be great!<br /> Please email feedback to info [@] gibthon.org, and click on the image to the left to get started.<br /><br />
+<html><div style="float:left; padding:0px 10px;"><img src="http://www.gibthon.org/images/gibthon.png" style="height:200px;" /></div><div style="height:180px;"><h3>Gibthon Construct Designer</h3> is a software tool under active development to aid with the design of primers for Gibson Assembly. It currently comes in two flavours - Gibthon Beta, and Gibthon Construct Designer Beta.</div>
-(This is currently running on a temp server in my living room, so please be patient if it falls over every now and again - a proper one is on the way)</div></html>
+<h4>Gibthon Beta - <a href="http://www.gibthon.org/">here</a></h4>
+The more basic of the two tools, this will take as an input the sequences surrounding the join for which you wish to design primers. You can interactively adjust the length of the primers to tune the melting temperature of the DNA and check for secondary structures.
+<h4>Gibthon Construct Designer Beta - <a href="http://dev.gibthon.org:19980/">Here</a></h4>
+Gibthon's older brother, and much more powerful. With this, you can design your construct from its component genes and automatically generate the optimal primers. The tool has access to the Parts Registry Database, the NCBI's Nucleotide database, as well as accepting genbank formatted files from the user.
+</html>
 {{:Team:Cambridge/Templates/footerMinimal}}