"
Team:Cambridge/Synthesis
From 2010.igem.org
(→SNCA (c. 434 bp)) |
(→SNCA (c. 434 bp)) |
||
| Line 58: | Line 58: | ||
menmendenivvgpkpfypieegsagtqlrkymeryaklgaiaftnavtgvdysyaeylekscclgkalqnyglvvdgrialcsenceeffipviaglfigvgvaptneiytlrelvhslgiskptivfsskkgldkvitvqktvttiktivildskvdyrgyqcldtfikrntppgfqassfktvevdrkeqvalimnssgstglpkgvqlthentvtrfshardpiygnqvspgtavltvvpfhhgfgmfttlgylicgfrvvmltkfdeetflktlqdykctNvilvptlfailnksellnkydlsnlveiasggaplskevgeavarrfnlpgvrqgygltettsaiiitpegddkpgasgkvvplfkakvidldtkkslgpnrrgevcvkgpmlmkgyvnnpeatkelideegwlhtgdigyydeekhffivdrlkslikykgyqvppaelesvllqhpsifdagvagvpdpvagelpgavvvlesgknmtekevmdyvasqvsnakrlrggvrfvdevpkgltgkidgraireilkkpvakm | menmendenivvgpkpfypieegsagtqlrkymeryaklgaiaftnavtgvdysyaeylekscclgkalqnyglvvdgrialcsenceeffipviaglfigvgvaptneiytlrelvhslgiskptivfsskkgldkvitvqktvttiktivildskvdyrgyqcldtfikrntppgfqassfktvevdrkeqvalimnssgstglpkgvqlthentvtrfshardpiygnqvspgtavltvvpfhhgfgmfttlgylicgfrvvmltkfdeetflktlqdykctNvilvptlfailnksellnkydlsnlveiasggaplskevgeavarrfnlpgvrqgygltettsaiiitpegddkpgasgkvvplfkakvidldtkkslgpnrrgevcvkgpmlmkgyvnnpeatkelideegwlhtgdigyydeekhffivdrlkslikykgyqvppaelesvllqhpsifdagvagvpdpvagelpgavvvlesgknmtekevmdyvasqvsnakrlrggvrfvdevpkgltgkidgraireilkkpvakm | ||
| - | + | ||
| - | + | ||
==Rcd aptamer construct (267 bp)== | ==Rcd aptamer construct (267 bp)== | ||
Latest revision as of 16:12, 2 August 2010
DNA Synthesis
LRE (c. 1000bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGGGCCAGTTGTTGAAAAAATTGCAGAACTTGGCAAGTATACGGTTGGAGAAGGTCCTCACTGGGATC ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT TTTGTAGTCAGTTTGGAACGTGAAATAGCCATTCTTACATGGGATGGCGTTAGTGCTGCACCTACAAGCA TAGAAGCTATTGTTAATGTCGAACCACACATTAAAAATAACAGACTCAATGATGGCAAAGCAGATCCCCT TGGCAATCTATGGACAGGTACAATGGCTATTGACGCTGGTCTCCCCGTAGGACCGGTCACTGGCAGTTTA TATCATTTAGGGGCTGATAAAAAGGTAAAAATGCACGAGAGCAACATAGCTATAGCAAATGGGCTCGCGT GGAGTAATGATTTGAAGAAAATGTATTATATTGATTCGGGGAAAAGAAGAGTAGACGAGTACGATTATGA TGCTTCTACATTATCCATCAGCAATCAACGGCCATTATTTACTTTTGAAAAGCATGAAGTGCCTGGATAT CCAGATGGTCAAACAATTGATGAGGAGGGTAATTTATGGGTTGCCGTTTTCCAAGGACAGCGAATTATTA AAATCAGTACCCAACAACCGGAAGTGTTACTGGATACCGTAAAAATACCAGATCCTCAGGTCACCTCTGT AGCATTTGGCGGTCCGAATTTGGATGAACTGCATGTAACATCTGCTGGTCTTCAGCTTGACGACAGTTCT TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG TTAAAGTGAAGCTATGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
EPIC Luciferase (c. 1764 bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAG AGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATAT CGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATAT GGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGG GCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAA AAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGA TGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGA TCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTT CCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGG ATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTAC AAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAAT ACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGT TGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTG ATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTG TGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCT GGAGACTTAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGGCCGCTTGAAGTCTTTAATTAAAT ACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGC GGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGA AAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCG GAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACGCGACGCAAGAAAAATCAGAGA GATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
Luciola cruciata orange
menmendenivvgpkpfypieegsagtqlrkymeryaklgaiaftnavtgvdysyaeylekscclgkalqnyglvvdgrialcsenceeffipviaglfigvgvaptneiytlrelvhslgiskptivfsskkgldkvitvqktvttiktivildskvdyrgyqcldtfikrntppgfqassfktvevdrkeqvalimnssgstglpkgvqlthentvtrfshardpiygnqvspgtavltvvpfhhgfgmfttlgylicgfrvvmltkfdeetflktlqdykctNvilvptlfailnksellnkydlsnlveiasggaplskevgeavarrfnlpgvrqgygltettsaiiitpegddkpgasgkvvplfkakvidldtkkslgpnrrgevcvkgpmlmkgyvnnpeatkelideegwlhtgdigyydeekhffivdrlkslikykgyqvppaelesvllqhpsifdagvagvpdpvagelpgavvvlesgknmtekevmdyvasqvsnakrlrggvrfvdevpkgltgkidgraireilkkpvakm
Rcd aptamer construct (267 bp)
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
cI857 (c. 714 bp)
http://openwetware.org/images/9/93/CI857_harvard_part.txt
Rcd (70 bp)
PPDK (2.6 kbp)
