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Team:Cambridge/Synthesis
From 2010.igem.org
(New page: {{:Team:Cambridge/Templates/header}} =DNA Synthesis= ==LRE== <html><span style="background-color:blue">gaattcgcggccgcttctagag</span></html> <html><span style="background-color:green">ttaag...) |
(→SNCA (c. 434 bp)) |
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| (10 intermediate revisions not shown) | |||
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{{:Team:Cambridge/Templates/header}} | {{:Team:Cambridge/Templates/header}} | ||
=DNA Synthesis= | =DNA Synthesis= | ||
| - | ==LRE== | + | ==LRE (c. 1000bp)== |
| - | <html><span style="background-color: | + | <html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> |
| - | <html><span style="background-color: | + | <html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> |
| - | <html><span style="background-color: | + | <html><span style="background-color:#FFE673">ATGGGGCCAGTTGTTGAAAAAATTGCAGAACTTGGCAAGTATACGGTTGGAGAAGGTCCTCACTGGGATC |
ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA | ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA | ||
AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT | AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT | ||
| Line 18: | Line 18: | ||
TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG | TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG | ||
TTAAAGTGAAGCTATGA</span></html> | TTAAAGTGAAGCTATGA</span></html> | ||
| - | <html><span style="background-color: | + | |
| + | <html><span style="background-color:#FE7276">gaattcgcggccgcttctag</span></html> | ||
| + | |||
| + | <html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | ||
| + | |||
| + | ==EPIC Luciferase (c. 1764 bp)== | ||
| + | <html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> | ||
| + | <html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> | ||
| + | <html><span style="background-color:#FFE673">ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAG | ||
| + | AGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATAT | ||
| + | CGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATAT | ||
| + | GGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGG | ||
| + | GCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG | ||
| + | TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAA | ||
| + | AAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGA | ||
| + | TGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGA | ||
| + | TCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTT | ||
| + | CCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGG | ||
| + | ATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT | ||
| + | ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTAC | ||
| + | AAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAAT | ||
| + | ACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGT | ||
| + | TGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTG | ||
| + | ATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTG | ||
| + | TGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT | ||
| + | TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCT | ||
| + | GGAGACTTAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGGCCGCTTGAAGTCTTTAATTAAAT | ||
| + | ACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGC | ||
| + | GGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGA | ||
| + | AAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCG | ||
| + | GAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACGCGACGCAAGAAAAATCAGAGA | ||
| + | GATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTGA</span></html> | ||
| + | |||
| + | <html><span style="background-color:#FE7276">gaattcgcggccgcttctag</span></html> | ||
| + | |||
| + | <html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | ||
| + | |||
| + | ==Luciola cruciata orange== | ||
| + | menmendenivvgpkpfypieegsagtqlrkymeryaklgaiaftnavtgvdysyaeylekscclgkalqnyglvvdgrialcsenceeffipviaglfigvgvaptneiytlrelvhslgiskptivfsskkgldkvitvqktvttiktivildskvdyrgyqcldtfikrntppgfqassfktvevdrkeqvalimnssgstglpkgvqlthentvtrfshardpiygnqvspgtavltvvpfhhgfgmfttlgylicgfrvvmltkfdeetflktlqdykctNvilvptlfailnksellnkydlsnlveiasggaplskevgeavarrfnlpgvrqgygltettsaiiitpegddkpgasgkvvplfkakvidldtkkslgpnrrgevcvkgpmlmkgyvnnpeatkelideegwlhtgdigyydeekhffivdrlkslikykgyqvppaelesvllqhpsifdagvagvpdpvagelpgavvvlesgknmtekevmdyvasqvsnakrlrggvrfvdevpkgltgkidgraireilkkpvakm | ||
| + | |||
| + | |||
| + | |||
| + | ==Rcd aptamer construct (267 bp)== | ||
| + | AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG | ||
| + | GUGCCUACAAU | ||
| + | ==cI857 (c. 714 bp)== | ||
| + | http://openwetware.org/images/9/93/CI857_harvard_part.txt | ||
| + | |||
| + | ==Rcd (70 bp)== | ||
| + | ==PPDK (2.6 kbp)== | ||
| + | |||
| + | |||
| + | |||
{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} | ||
Latest revision as of 16:12, 2 August 2010
DNA Synthesis
LRE (c. 1000bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGGGCCAGTTGTTGAAAAAATTGCAGAACTTGGCAAGTATACGGTTGGAGAAGGTCCTCACTGGGATC ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT TTTGTAGTCAGTTTGGAACGTGAAATAGCCATTCTTACATGGGATGGCGTTAGTGCTGCACCTACAAGCA TAGAAGCTATTGTTAATGTCGAACCACACATTAAAAATAACAGACTCAATGATGGCAAAGCAGATCCCCT TGGCAATCTATGGACAGGTACAATGGCTATTGACGCTGGTCTCCCCGTAGGACCGGTCACTGGCAGTTTA TATCATTTAGGGGCTGATAAAAAGGTAAAAATGCACGAGAGCAACATAGCTATAGCAAATGGGCTCGCGT GGAGTAATGATTTGAAGAAAATGTATTATATTGATTCGGGGAAAAGAAGAGTAGACGAGTACGATTATGA TGCTTCTACATTATCCATCAGCAATCAACGGCCATTATTTACTTTTGAAAAGCATGAAGTGCCTGGATAT CCAGATGGTCAAACAATTGATGAGGAGGGTAATTTATGGGTTGCCGTTTTCCAAGGACAGCGAATTATTA AAATCAGTACCCAACAACCGGAAGTGTTACTGGATACCGTAAAAATACCAGATCCTCAGGTCACCTCTGT AGCATTTGGCGGTCCGAATTTGGATGAACTGCATGTAACATCTGCTGGTCTTCAGCTTGACGACAGTTCT TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG TTAAAGTGAAGCTATGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
EPIC Luciferase (c. 1764 bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAG AGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATAT CGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATAT GGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGG GCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAA AAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGA TGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGA TCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTT CCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGG ATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTAC AAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAAT ACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGT TGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTG ATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTG TGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCT GGAGACTTAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGGCCGCTTGAAGTCTTTAATTAAAT ACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGC GGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGA AAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCG GAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACGCGACGCAAGAAAAATCAGAGA GATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
Luciola cruciata orange
menmendenivvgpkpfypieegsagtqlrkymeryaklgaiaftnavtgvdysyaeylekscclgkalqnyglvvdgrialcsenceeffipviaglfigvgvaptneiytlrelvhslgiskptivfsskkgldkvitvqktvttiktivildskvdyrgyqcldtfikrntppgfqassfktvevdrkeqvalimnssgstglpkgvqlthentvtrfshardpiygnqvspgtavltvvpfhhgfgmfttlgylicgfrvvmltkfdeetflktlqdykctNvilvptlfailnksellnkydlsnlveiasggaplskevgeavarrfnlpgvrqgygltettsaiiitpegddkpgasgkvvplfkakvidldtkkslgpnrrgevcvkgpmlmkgyvnnpeatkelideegwlhtgdigyydeekhffivdrlkslikykgyqvppaelesvllqhpsifdagvagvpdpvagelpgavvvlesgknmtekevmdyvasqvsnakrlrggvrfvdevpkgltgkidgraireilkkpvakm
Rcd aptamer construct (267 bp)
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
cI857 (c. 714 bp)
http://openwetware.org/images/9/93/CI857_harvard_part.txt
Rcd (70 bp)
PPDK (2.6 kbp)
