"
Team:Cambridge/Synthesis
From 2010.igem.org
(→EPIC Luciferase) |
(→LRE) |
||
| Line 1: | Line 1: | ||
{{:Team:Cambridge/Templates/header}} | {{:Team:Cambridge/Templates/header}} | ||
=DNA Synthesis= | =DNA Synthesis= | ||
| - | ==LRE== | + | ==LRE (c. 1000bp)== |
<html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> | <html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> | ||
<html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> | <html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> | ||
Revision as of 14:13, 29 July 2010
DNA Synthesis
LRE (c. 1000bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGGGCCAGTTGTTGAAAAAATTGCAGAACTTGGCAAGTATACGGTTGGAGAAGGTCCTCACTGGGATC ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT TTTGTAGTCAGTTTGGAACGTGAAATAGCCATTCTTACATGGGATGGCGTTAGTGCTGCACCTACAAGCA TAGAAGCTATTGTTAATGTCGAACCACACATTAAAAATAACAGACTCAATGATGGCAAAGCAGATCCCCT TGGCAATCTATGGACAGGTACAATGGCTATTGACGCTGGTCTCCCCGTAGGACCGGTCACTGGCAGTTTA TATCATTTAGGGGCTGATAAAAAGGTAAAAATGCACGAGAGCAACATAGCTATAGCAAATGGGCTCGCGT GGAGTAATGATTTGAAGAAAATGTATTATATTGATTCGGGGAAAAGAAGAGTAGACGAGTACGATTATGA TGCTTCTACATTATCCATCAGCAATCAACGGCCATTATTTACTTTTGAAAAGCATGAAGTGCCTGGATAT CCAGATGGTCAAACAATTGATGAGGAGGGTAATTTATGGGTTGCCGTTTTCCAAGGACAGCGAATTATTA AAATCAGTACCCAACAACCGGAAGTGTTACTGGATACCGTAAAAATACCAGATCCTCAGGTCACCTCTGT AGCATTTGGCGGTCCGAATTTGGATGAACTGCATGTAACATCTGCTGGTCTTCAGCTTGACGACAGTTCT TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG TTAAAGTGAAGCTATGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
EPIC Luciferase (c. 1764 bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAG AGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATAT CGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATAT GGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGG GCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAA AAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGA TGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGA TCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTT CCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGG ATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTAC AAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAAT ACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGT TGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTG ATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTG TGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCT GGAGACTTAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGGCCGCTTGAAGTCTTTAATTAAAT ACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGC GGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGA AAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCG GAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACGCGACGCAAGAAAAATCAGAGA GATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
SNCA (c. 434 bp)
http://www.genome.jp/dbget-bin/www_bget?hsa:6622
Rcd aptamer construct (267 bp)
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
