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Team:Cambridge/Synthesis
From 2010.igem.org
(→SNCA) |
(→EPIC Luciferase) |
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| Line 23: | Line 23: | ||
<html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | <html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | ||
| - | ==EPIC Luciferase== | + | ==EPIC Luciferase (c. 1764 bp)== |
<html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> | <html><span style="background-color:#6EE768">gaattcgcggccgcttctagag</span></html> | ||
<html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> | <html><span style="background-color:#866FD7">ttaaggaggtaaaaaaa</span></html> | ||
| Line 54: | Line 54: | ||
<html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | <html><span style="background-color:#6EE768">Biobrick prefix</span></html><html><span style="background-color:#866FD7">RBS</span></html><html><span style="background-color:#FFE673">Coding sequence to be optimised for E. coli</span></html><html><span style="background-color:#FE7276">BioBrick suffix</span></html> | ||
| + | |||
==SNCA (c. 434 bp)== | ==SNCA (c. 434 bp)== | ||
http://www.genome.jp/dbget-bin/www_bget?hsa:6622 | http://www.genome.jp/dbget-bin/www_bget?hsa:6622 | ||
Revision as of 14:13, 29 July 2010
DNA Synthesis
LRE
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGGGCCAGTTGTTGAAAAAATTGCAGAACTTGGCAAGTATACGGTTGGAGAAGGTCCTCACTGGGATC ATGAAACTCAGACCTTATATTTCGTCGACACCGTAGAGAAAACTTTTCATAAATATGTACCTTCTCAGAA AAAATACACGTTTTGTAAAGTAGATAAACTGGTTTCTTTCATTATTCCCCTTGCTGGATCCCCTGGCCGT TTTGTAGTCAGTTTGGAACGTGAAATAGCCATTCTTACATGGGATGGCGTTAGTGCTGCACCTACAAGCA TAGAAGCTATTGTTAATGTCGAACCACACATTAAAAATAACAGACTCAATGATGGCAAAGCAGATCCCCT TGGCAATCTATGGACAGGTACAATGGCTATTGACGCTGGTCTCCCCGTAGGACCGGTCACTGGCAGTTTA TATCATTTAGGGGCTGATAAAAAGGTAAAAATGCACGAGAGCAACATAGCTATAGCAAATGGGCTCGCGT GGAGTAATGATTTGAAGAAAATGTATTATATTGATTCGGGGAAAAGAAGAGTAGACGAGTACGATTATGA TGCTTCTACATTATCCATCAGCAATCAACGGCCATTATTTACTTTTGAAAAGCATGAAGTGCCTGGATAT CCAGATGGTCAAACAATTGATGAGGAGGGTAATTTATGGGTTGCCGTTTTCCAAGGACAGCGAATTATTA AAATCAGTACCCAACAACCGGAAGTGTTACTGGATACCGTAAAAATACCAGATCCTCAGGTCACCTCTGT AGCATTTGGCGGTCCGAATTTGGATGAACTGCATGTAACATCTGCTGGTCTTCAGCTTGACGACAGTTCT TTGGACAAAAGTTTAGTTAATGGGCACGTCTACAGAGTAACAGGTTTAGGCGTCAAAGGTTTCGCGGGAG TTAAAGTGAAGCTATGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
EPIC Luciferase (c. 1764 bp)
gaattcgcggccgcttctagag ttaaggaggtaaaaaaa ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAG AGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATAT CGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATAT GGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGG GCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAA AAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGA TGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGA TCGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTT CCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGG ATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTAC AAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAAT ACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGT TGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTG ATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTG TGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCT GGAGACTTAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGGCCGCTTGAAGTCTTTAATTAAAT ACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGC GGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGA AAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCG GAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACGCGACGCAAGAAAAATCAGAGA GATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTGA
gaattcgcggccgcttctag
Biobrick prefixRBSCoding sequence to be optimised for E. coliBioBrick suffix
SNCA (c. 434 bp)
http://www.genome.jp/dbget-bin/www_bget?hsa:6622
Rcd aptamer construct (267 bp)
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
