Team:Cambridge/References/ProjectBioluminescence/Luciferase
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(→Mutant (EPIC) luciferase (P. pyralis)) |
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*[[Team:Cambridge/ProjectBioluminescence/Luciferase/Notes| Notes]] | *[[Team:Cambridge/ProjectBioluminescence/Luciferase/Notes| Notes]] | ||
*[http://partsregistry.org/Lux The lux wiki from parts registry] | *[http://partsregistry.org/Lux The lux wiki from parts registry] | ||
- | + | *[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters" | |
- | + | *[http://partsregistry.org/wiki/index.php/Part:BBa_I712019 The Firefly Luciferase in the registry]. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters" | |
*[http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence] | *[http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence] | ||
- | *[http://partsregistry.org/Part: | + | *[http://partsregistry.org/wiki/index.php/Part:BBa_I712052 Parts Registry Luciferase under control of Phage promoter] - T7 Phage |
*[[Team:Cambridge/ProjectBioluminescence/Luciferase/IMPORTANT INFO | IMPORTANT INFO]] | *[[Team:Cambridge/ProjectBioluminescence/Luciferase/IMPORTANT INFO | IMPORTANT INFO]] | ||
Revision as of 10:12, 23 July 2010
Mutant (EPIC) luciferase (P. pyralis)
- Mutant (EPIC) Luciferase (P.pyralis) Sequence
- Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- 2006 attempts at BioBricking the lux operon
- Notes
- The lux wiki from parts registry
- The Firefly Luciferase in the registry. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
- The Firefly Luciferase in the registry. There is no promotor added onto it presumed by the phrase "acts as a reporter protein when connected to other proteins or promoters"
- NCBI Lux operon sequence
- Parts Registry Luciferase under control of Phage promoter - T7 Phage
- IMPORTANT INFO
Plasmid experiment
- Vibrio plasmid experiment -possible source of lux operon
- [1] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?