Team:Cambridge/Quiescence/Sequences

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Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:

AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUcuuggagcugaugagccguucgcggcgaaaacu aAGCUUAUUGCUugguuacuccaagAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCA UUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCGGUGCCUACAAU

This sequence does not include an aptamer.

Including aptamer

AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU

Rcd sequences

Rcd 70

AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd 95

AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG AACGCGUUUUAGCGGUGCCUACAAU

Constructs

Rcd70_aptazyme_theo+_30_3 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUUGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd70_aptazyme_theo+_28_9 CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd70_aptazyme_theo+_30_8 CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG