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Team:Cambridge/Quiescence/Sequences
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Peteremmrich (Talk | contribs) (→Biobricked construct for assembly from oligos) |
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Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | ||
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GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU | GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU | ||
UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
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| + | ==Biobricked construct for assembly from oligos== | ||
| + | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA | ||
| + | AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG | ||
| + | AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG | ||
| + | TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG | ||
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| + | ===PCR Oligo Maker output=== | ||
| + | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA | ||
| + | GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG | ||
| + | CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG | ||
| + | GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG | ||
| + | GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC | ||
| + | CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG | ||
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Latest revision as of 11:09, 13 August 2010
Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUcuuggagcugaugagccguucgcggcgaaaacu aAGCUUAUUGCUugguuacuccaagAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCA UUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCGGUGCCUACAAU
This sequence does not include an aptamer.
Including aptamer
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
Rcd sequences
Rcd 70
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd 95
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG AACGCGUUUUAGCGGUGCCUACAAU
Constructs
Rcd70_aptazyme_theo+_30_3 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_28_9 CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_30_8 CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Biobricked construct for assembly from oligos
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG
PCR Oligo Maker output
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG
