Team:Cambridge/Quiescence/Sequences
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Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | ||
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Rcd70_aptazyme_theo+_30_3 | Rcd70_aptazyme_theo+_30_3 | ||
gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU | gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU | ||
- | CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA| | + | CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU |
+ | GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
Rcd70_aptazyme_theo+_28_9 | Rcd70_aptazyme_theo+_28_9 | ||
- | CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc| | + | CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC |
- | + | UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC | |
+ | GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
Rcd70_aptazyme_theo+_30_8 | Rcd70_aptazyme_theo+_30_8 | ||
- | CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc| | + | CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU |
+ | GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU | ||
UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
- | {{:Team:Cambridge/Templates/ | + | |
+ | ==Biobricked construct for assembly from oligos== | ||
+ | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA | ||
+ | AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG | ||
+ | AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG | ||
+ | TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG | ||
+ | |||
+ | ===PCR Oligo Maker output=== | ||
+ | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA | ||
+ | GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG | ||
+ | CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG | ||
+ | GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG | ||
+ | GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC | ||
+ | CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG | ||
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Latest revision as of 11:09, 13 August 2010
Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUcuuggagcugaugagccguucgcggcgaaaacu aAGCUUAUUGCUugguuacuccaagAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCA UUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCGGUGCCUACAAU
This sequence does not include an aptamer.
Including aptamer
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
Rcd sequences
Rcd 70
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd 95
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG AACGCGUUUUAGCGGUGCCUACAAU
Constructs
Rcd70_aptazyme_theo+_30_3 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_28_9 CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_30_8 CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Biobricked construct for assembly from oligos
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG
PCR Oligo Maker output
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG