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Team:Cambridge/Notebook/Week3
From 2010.igem.org
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Revision as of 20:21, 28 July 2010
Monday
Afternoon
- Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
- Anja, Hannah and Emily worked on text for Sterilin
- Peter and Will created primer sequences for first experiment
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
