Team:Cambridge/Gibson/Introduction

From 2010.igem.org

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* Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] for ligating two fragments, Gibson is perfect for doing multi part systems, since it takes the same amount of time to ligate n pieces of DNA together as it does for 2 pieces.
* Although it is roughly the same speed as [http://partsregistry.org/Help:BioBrick_Assembly standard BioBrick assembly] for ligating two fragments, Gibson is perfect for doing multi part systems, since it takes the same amount of time to ligate n pieces of DNA together as it does for 2 pieces.
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<html>
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{|class="wikitable"
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<style>
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|-
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table.vistable td{border-right:1px solid gray; border-top:1px solid gray; padding:10px;}
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|'''Assembly Method'''
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table.vistable{border-left:1px solid gray; border-bottom:1px solid gray;}
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|'''Number of steps to ligate N pieces of DNA'''
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</style>
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|-
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<div align="center">
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|[http://partsregistry.org/Assembly:Standard_assembly Standard Assembly]
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<table class="vistable" padding="0" cellspacing="0">
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|[[Image:Steps-N.png]]
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|-
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<tr>
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|[http://partsregistry.org/Assembly:Rolling_assembly Parallel Assembly]
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<td><b>Assembly Method</b>
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|[[Image:Steps-Log2.png]] (rounded up)
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</td><td><b>Number of steps to ligate N pieces of DNA</b>
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|-
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</td></tr>
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|[http://2010.igem.org/Team:Cambridge/Gibson/Mechanism Gibson Assembly]
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<tr>
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|[[Image:Steps1.png]]
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<td><a href="http://partsregistry.org/Assembly:Standard_assembly" class="external text" title="http://partsregistry.org/Assembly:Standard_assembly" rel="nofollow">Standard Assembly</a>
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|}
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</td><td><a href="/Image:Steps-N.png" class="image" title="Image:Steps-N.png"><img alt="Image:Steps-N.png" src="/wiki/images/c/cc/Steps-N.png" width="92" height="18" border="0" /></a>
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</td></tr>
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<tr>
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<td><a href="http://partsregistry.org/Assembly:Rolling_assembly" class="external text" title="http://partsregistry.org/Assembly:Rolling_assembly" rel="nofollow">Parallel Assembly</a>
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</td><td><a href="/Image:Steps-Log2.png" class="image" title="Image:Steps-Log2.png"><img alt="Image:Steps-Log2.png" src="/wiki/images/6/6f/Steps-Log2.png" width="138" height="21" border="0" /></a> (rounded up)
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</td></tr>
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<tr>
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<td><a href="http://2010.igem.org/Team:Cambridge/Gibson/Mechanism" class="external text" title="http://2010.igem.org/Team:Cambridge/Gibson/Mechanism" rel="nofollow">Gibson Assembly</a>
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</td><td><a href="/Image:Steps1.png" class="image" title="Image:Steps1.png"><img alt="Image:Steps1.png" src="/wiki/images/1/10/Steps1.png" width="83" height="18" border="0" /></a>
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</td></tr></table>
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</div>
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</html>
== Disadvantages ==
== Disadvantages ==

Revision as of 13:13, 21 October 2010