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Team:Cambridge/BioluminescenceLinks
From 2010.igem.org
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*[http://sciencelinks.jp/j-east/article/200514/000020051405A0424228.php Conversion of normal luciferin into firefly luciferin] | *[http://sciencelinks.jp/j-east/article/200514/000020051405A0424228.php Conversion of normal luciferin into firefly luciferin] | ||
* Don't use luciferinol, it r teh suckage | * Don't use luciferinol, it r teh suckage | ||
| + | * [http://jeb.biologists.org/cgi/reprint/137/1/39.pdf Glowing fish what?] | ||
==Will's section== | ==Will's section== | ||
Revision as of 15:19, 15 July 2010
Bioluminescence
Emily's Section
- Metridia luciferase - requires CBP Protein
- Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
- CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586
- CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf
- Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330
- Luciferin Regenerating Enzyme (LRE) - LRE (in Photinus pyralis)
- Regeneration of Aequorin in Aequorea Aequorea jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
- Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051
- Light Emission Quanta - Spectral Emission and Quantum Yield of Firefly Bioluminescence
- One light quantum emitted for every luciferin molecule oxidized
- CoA stimulation - CoA in Luminescence
- Bacterial luciferase reaction - Bacterial Luciferin
Theo's section
- Self contained part for Photorhabdus luminescens lux operon: http://partsregistry.org/Part:BBa_J70008:Design
- Firefly diagram from Emily's paper:
- Fireflow luciferin normally emits at 560nm but at higher temperatures at 630nm
- http://pubs.acs.org/doi/pdf/10.1021/bi00702a015 Kinetics
- Mg2+ required
- A nice background summary: http://irishmarinelife.com/bioluminescence-symbioses/
- The guy who made the biobrick's logbook: http://openwetware.org/wiki/User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick/2008/04/14
Vibrio
- luxC = acyl coA reductase
Bill's section
- Conversion of normal luciferin into firefly luciferin
- Don't use luciferinol, it r teh suckage
- Glowing fish what?
Will's section
Anja's section
- Paper on mutant luciferase with increased activity
- Wavelength= 469 nm
- Low leakage rate
- Does not disrupt cell functions/ devt.
- Requires continual addition of luciferin
Peter's section
Ben's section
Flashlight fish symbiont luciferase genes have been sequenced but cannot be sucessfully expressed in E. Coli
