Team:Cambridge/Bioluminescence/Luciola cruciata

Photinus pyralis (source)

The Japanese firefly, Luciola cruciata is notable in that nearby fireflies flash in synchrony, making the trees pulsate with green light which fishermen use as a guide to bring them home. The Cambridge team selected Luciola cruciata because Kajiyama and Nakano (1991) had described a suite of single amino acid changes which significantly changed the wavelength of peak emission.

Site directed mutagenesis with Gibson Assembly