Team:Cambridge/Bioluminescence/G28

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{{:Team:Cambridge/Templates/headerbar|colour=#386abc|caption=Project Vibrio: The LuxBrick}}
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{{:Team:Cambridge/Templates/RightImage|image=Space_invader.jpg|caption=The LuxBrick on a 96 well plate}}
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Whilst researching the literature on bioluminescence at the beginning of the summer, we came across a report on [http://departments.kings.edu/biology/lux/bacterial.html 'Molecular Biology Experiments Utilizing the lux Genes of ''Vibrio fischeri'''] by James Slock from King's College, PA. Dr Slock kindly provided us the two plasmids mentioned in these experiments, which carry the complete ''V. fischeri'' lux operon (LuxICDABE regulated by Lux R). Using Long-Range PCR, we extracted luxCD, luxAB and luxE individually and assembled them into a new operon. As described in the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background Background section], lux I and lux R exert tight quorum sensing control on expression of the lux operon in ''V. fischeri''. In the absence of LuxR protein and AHL the Lux genes are virtually inactive.
Whilst researching the literature on bioluminescence at the beginning of the summer, we came across a report on [http://departments.kings.edu/biology/lux/bacterial.html 'Molecular Biology Experiments Utilizing the lux Genes of ''Vibrio fischeri'''] by James Slock from King's College, PA. Dr Slock kindly provided us the two plasmids mentioned in these experiments, which carry the complete ''V. fischeri'' lux operon (LuxICDABE regulated by Lux R). Using Long-Range PCR, we extracted luxCD, luxAB and luxE individually and assembled them into a new operon. As described in the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background Background section], lux I and lux R exert tight quorum sensing control on expression of the lux operon in ''V. fischeri''. In the absence of LuxR protein and AHL the Lux genes are virtually inactive.

Latest revision as of 23:02, 27 October 2010