Team:Cambridge/Bioluminescence/Firefly Modelling

From 2010.igem.org

(Difference between revisions)
Line 2: Line 2:
{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Modelling}}
{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Modelling}}
-
We modelled the Luciferin cycle as described in the [http://www.ncbi.nlm.nih.gov/pubmed/18949818 literature] We used data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye]'s review and the paper on [http://www.ncbi.nlm.nih.gov/pubmed/20655239 firefly luciferase inhibition] from J.M Leitão ''et al.''
+
We modelled the Luciferin cycle as described by [http://www.ncbi.nlm.nih.gov/pubmed/18949818 Marques 2009 and others. We were assisted by data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye (2010)] and [http://www.ncbi.nlm.nih.gov/pubmed/20655239 J.M Leitão ''et al.'' (2010)] on the inhibition of firefly luciferase.
We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below:  
We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below:  
Line 8: Line 8:
[[Image:Modelluccycle.jpg|center|700px]]
[[Image:Modelluccycle.jpg|center|700px]]
-
Some of the parameters could be found in the litterature, but some of the parameters (namely the diffusion of Luciferin into the cell and the regeneration rate were inferred from experimental data.)  
+
Some parameters were taken from literature.  However there was no clear data for others, such that we were only able to define some parameters in the light of experimental data.
 +
 
 +
While attempting to optimise our system we modelled the effect of [http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferin Regenerating Enzyme (LRE)]
 +
 
We modelled the system both with and without the effect of the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferase Regenerating Enzyme] (LRE.) The 4 Figures below show a comparison the model and our data for both cases.   
We modelled the system both with and without the effect of the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferase Regenerating Enzyme] (LRE.) The 4 Figures below show a comparison the model and our data for both cases.   
 +
[[Image:Cambridge-LREmodel.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']]
 +
Our model suggested that the LRE would make light output increase faster, peak higher and decay more slowly.  These were all qualities which suited us.
[[Image:Modellight.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']]
[[Image:Modellight.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']]

Revision as of 01:57, 27 October 2010