Thursday August 5, 2010

Dev

OBTAINED COLONIES! Six colonies from each of the four plates were chosen to be tested for the correct construct (K135000+I13504 or K135000+I13507) by means of a colony PCR.

Raida

Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes.

Chris

Today, I spent the day contacting sponsors and preparing the sponsorship letters that would be used to contact the different faculties. I found colonies of the pSB1AC3 and pSB1AK3 from the registry and made overnight cultures to be Miniprepped tomorrow. Finally, I began the digest of the CpxP promoter in preparation of inserting it into a plasmid.