Monday July 26, 2010

Chris's Agarose Gel Electrophoresis of the regular PCR of the CpxP promoter. The bands are very nonspecific, indicating that the DNA was not amplified.

Jeremy

To do: I13504 K239000 Miniprep C3, I13507 K239000 Miniprep all, I13504 K135000 Redo, I13507 K135000 I13504 K239000(plasmid switch) and PCR, I13507+K135000 C3,C4,C6.

PCR sequencing -order primers, gradient PCR

Dev

Today, I worked on the construction of K135000 with I13504.

Chris

Today, I ran agarose gel electrophoresis of the different CpxP promoters that were put into Ampicillin-chloramphenicol and ampicillin-kanamycin plasmids. The first gel that was run was of the promoters having been digested with the restriction enzymes XbaI and PstI. This gel can be seen on the right. The result of the gel showed bands around approximately 3000 bp, meaning tht the plasmid was not cyt by the enzymes. The second gel that was run of the CpxP promoter after a regular PCR with Biobrick primers. The bands were very inconclusive and thus, we cannot conclude that the CpxP promoter is present.