Dev's gel of restriction digest of E0040+B0015

Thursday June 24, 2010

Emily

Today primers came in for the maltose binding protein. After diluting them, we set up a PCR with a variety of different colonies in order to try to PCR this gene about of the genome of E Coli.

Patrick

The previous day's R0040-E0430 and R0040-E0420 constructs grew colonies, but there was no fluorescence visible. I will leave this for another day just to see. As well, I selected five colonies from each plate of cpxP in pSB1A3. They have been put into an overnight culture for miniprepping tomorrow.

Dev

Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the 4000-3000 range which could be uncut plasmids