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Team:Calgary/23 June 2010
From 2010.igem.org
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'''Wednesday June 23, 2010''' | '''Wednesday June 23, 2010''' | ||
| - | Patrick | + | |
| + | <u>Patrick</u> | ||
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| + | Today, I did a restriction digest in order to construct the ''cpxP'' PCR product into pSB1A3 plasmid. This was done with ''XbaI'' and ''SpeI'' as those were the only sites on the PCR product. This will result in unintentional scars, but we will hopefully deal with this later on. Along with this, I also constructed an R0040 promoter in front of both E0430 and E0420 (switched onto pSB1K2). | ||
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Revision as of 18:50, 7 July 2010
Emily's PCR Gel
Wednesday June 23, 2010
Patrick
Today, I did a restriction digest in order to construct the cpxP PCR product into pSB1A3 plasmid. This was done with XbaI and SpeI as those were the only sites on the PCR product. This will result in unintentional scars, but we will hopefully deal with this later on. Along with this, I also constructed an R0040 promoter in front of both E0430 and E0420 (switched onto pSB1K2).
No notebook page exists for this date. Sorry!
