Team:Calgary/15 July 2010

From 2010.igem.org

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'''Thurday July 15, 2010'''
'''Thurday July 15, 2010'''
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''RAIDA:''
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<u>Raida</u>
*Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.  
*Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.  
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*I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
*I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
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Revision as of 23:13, 15 July 2010

Thurday July 15, 2010

Raida

  • Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.

PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius

  • I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.

No notebook page exists for this date. Sorry!