Monday May 10, 2010

iGEM Begins!!

This was the first day that our entire team met to begin doing our project.

Today, we learned the dimensions needed for a Polymerase Chain Reaction (PCR) Master Mix (which can be found in Lab Protocols) and attempted a PCR of our own to multiply some DNA obtained from the registry. We used plasmids R0040 123502 (Red), R0040 GFP C2 (Blue) and R0040 GFP C1 (Yellow). In addition to this, we learned how to use agarose gel electrophoresis to determine whether or not the DNA was present from the PCR reaction.