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Team:Bielefeld-Germany/Project/Protocols
==Determination of minimal inhibitory concentration (MIC) of kanamycin== ...389212 (native ''virA'') in bacteria which are already containing K389101 (kanamycin resistance read out) using [[#Transformation via electroporation |electropo

30 KB (4,241 words) - 02:20, 28 October 2010
Team:Bielefeld-Germany/Project/Theory
...ts. Thus, we wanted to construct our system to lead in the expression of a kanamycin resistance after the induction of the VirA receptor, enabling the quick exc ... expression of aminoglycoside phosphotransferase (APH) that can inactivate kanamycin. The mode of inactivation is the transfer of the y-phosphate from ATP to th

35 KB (5,106 words) - 22:39, 27 October 2010
Team:Alberta/Notebook/BasePlasmids
Our original base plasmids contain a Kanamycin resistance cassette (p1003) bracketed by BsaI or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky

8 KB (1,183 words) - 01:09, 28 October 2010
Team:Alberta/Building Parts
...or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky ends characteristic of an A or a B BioByte. May 10, 2010 PCRed p1003 (Kanamycin cassette)with primers PrA_p1003+ and PrB'_p1003-

30 KB (4,641 words) - 19:55, 19 August 2010
Team:Harvard/vectors/parts
...d>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb" ...d>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.g

8 KB (1,499 words) - 23:19, 26 October 2010
Team:ULB-Brussels/Homologous
...sequences (fig.2). There are two resistance cassette plasmids, one with a kanamycin resistance marker (RCPKan) and the other with a chloramphenicol resistance <leg>Figure 2 : Resistance cassette kanamycin</leg>

19 KB (2,943 words) - 20:44, 27 October 2010
Team:Kyoto/Notebook1
... to LB plate (Amp+). And We didn't pre-culture B0015 despite its vector is Kanamycin-resistance. So, it was predicted that we will fail the transformation of pS |rowspan="2"|1||Kanamycin||○||rowspan="14"|At 37℃, 08/10 20:00-08/11 9:00

55 KB (7,098 words) - 02:17, 28 October 2010
Team:Bielefeld-Germany/Results/Characterization/K389011
...each plate were divided by the total number of colonies on a plate without kanamycin to calculate the survival rate φ. The ability of the bacterial populati ...ced population) indicate a common tendency. Under rising concentrations of kanamycin (x-axis) the survival rate of each population (y-axis) first decreases slow

4 KB (533 words) - 00:59, 28 October 2010
Team:LMU-Munich/Notebook/Apoptosis
- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin) - 3 ml LB-Media + 4 µl Kanamycin

92 KB (13,163 words) - 18:04, 27 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System
...B (Fig3-0-3), production of LuxI is repressed by LasR/3OC12HSL complex and kanamycin resistance gene is activated by LasR/3OC12HSL complex. This means that 3OC1 ...ntration of 3OC12HSL and 3OC6HSL is too low to activate the expressions of kanamycin resistance gene and chloramphenicol resistance gene, respectively. 

13 KB (2,086 words) - 03:59, 28 October 2010
2010.igem.org/Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,317 words) - 18:23, 22 July 2010
Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,354 words) - 03:02, 25 October 2010
Team:Bielefeld-Germany/Results/Used
|style="border-style: solid; border-width: 1px"| Kanamycin resistance gene, <partinfo>K389005</partinfo>: <partinfo>K389005 SpecifiedC ...pectively. It was isolated from the BioBrick <partinfo>P1003</partinfo> (a kanamycin resistance gene with promoter and RBS).

31 KB (4,237 words) - 21:36, 27 October 2010
Team:INSA-Lyon/Project/Stage2/Results
<li>double transformation with ampicillin and kanamycin</li> <li>The lipid part with kanamycin</li>

4 KB (625 words) - 00:36, 28 October 2010
Page79
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the

24 KB (3,715 words) - 22:22, 8 July 2010
Team:Alberta/Notebook May
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR Kanamycin resistance cassette fragment from p1003 containing either the A/B' ends or

31 KB (4,965 words) - 18:29, 4 August 2010
Team:Stanford/Notebook/Lab Work/Week 2
*Transferred 50uL of transformed cells to agar plate with kanamycin. ====Making Kanamycin, Ampicillin Chloramphenicol Plates====

11 KB (1,622 words) - 02:05, 14 September 2010
Team:Bielefeld-Germany/Results/Submitted
===Neomycin / kanamycin resistance=== ...orm. We will use the BioBrick <partinfo>P1003</partinfo> as source for the kanamycin resistance gene.

9 KB (1,365 words) - 02:07, 28 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/September
Next steps: Picking clones of the trafo plates (containing Kanamycin) and perform the Mini-Prep. The obtaining constructs finally will be fused ...rmed according to standard protocol using BL21 cells and plates containing Kanamycin.

196 KB (29,094 words) - 21:34, 27 October 2010
Team:Michigan/Oil Sands August September
''' Transformation of Flu Operon + Kanamycin Backbone Ligation''' ...incubate longer as the slow growth may be due to the high concentration of Kanamycin (50mg/mL) added to the plates.

34 KB (5,403 words) - 01:36, 27 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System/modeling
...al Cooperation System. Fourth, in the contrast, when the system worked and Kanamycin existed, the number of cell B increased after temporal decline (Fig.3-4-4). ...ch_kan_sim_result.jpg|300px|left|thumb|Fig3-4-4 when the system worked and Kanamycin existed (work done by Shohei Kitano) ]]

9 KB (1,421 words) - 03:58, 28 October 2010
Team:Tsinghua/project/outline/m1
In donor plasmid A, we insert only one gene, kanamycin resistant gene (Kanr) to test our recombination. At the 5’ end of Kanr, w In donor plasmid B, we insert two genes, kanamycin resistant gene (Kanr) and Chloromycetin resistant gene (Chlr). At the 5’

20 KB (3,173 words) - 16:15, 27 October 2010
Team:Kyoto/Parts
...23-L||[http://partsregistry.org/Part:BBa_pSB1AK3 BBa_pSB1AK3]||Ampicillin, Kanamycin||129||3189 ...Ba_J23101]||2-17-F||[http://partsregistry.org/Part:BBa_pSB3K3 BBa_pSB3K3]||Kanamycin||919||2750

9 KB (1,303 words) - 22:47, 27 October 2010
Team:Michigan/Quorum Sensing
7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to

19 KB (2,960 words) - 01:14, 27 October 2010
Team:Kyoto/Protocols
#* Kanamycin: #*; Kanamycin: 0.5g

19 KB (3,027 words) - 02:42, 28 October 2010
Team:Alberta/Notebook July
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis

30 KB (4,765 words) - 22:55, 10 August 2010
Team:Alberta/Notebook August
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis

26 KB (3,927 words) - 21:02, 10 August 2010
Team:Michigan/Alex's Notebook
7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to

20 KB (3,132 words) - 04:36, 4 October 2010
Team:Michigan/Pili August September
2. Added 5 microliters of Kanamycin to each of the 50 mL tubes in step 1. *Marc adds 50uL of kanamycin onto each LB-agar plate and spread evenly with sterile glass beads

12 KB (1,774 words) - 03:05, 27 October 2010
Team:NCTU Formosa/Temperature Control
...n the appropriate media and antibiotic, such as agar plates with 25 µg/ml kanamycin. ...2% casamino acids, 1 mM MgSO4, 2 μM thiamine, 10 mM glucose, and 50 ng/ml Kanamycin antibiotic.) 

22 KB (2,885 words) - 02:18, 28 October 2010
Team:Calgary/3 August 2010
...ot grow of both regular Top10 cells and ccdB resistant cells on ampicillin-kanamycin and ampicillin-chloramphenicol plates.

3 KB (587 words) - 03:01, 27 October 2010
Team:Waterloo
...tor for both the measured part and the standard is pSB3K3 (low copy number kanamycin resistant BioBrick plasmid) with green ﬂuorescent protein gene as reporte I20260 on pSB3K3 inocluated into Kanamycin LB 

152 KB (22,732 words) - 17:38, 23 November 2010
Team:Caltech/Week 2
...e kanamycin test grew bacteria, suggesting that there is an issue with the kanamycin stock, which we will have to remake. ** Set up antibiotic test plates for kanamycin (kan) & tetracycline (tet), just as on [https://2010.igem.org/Team:Caltech/

8 KB (1,258 words) - 19:00, 21 July 2010
Team:TU Delft/6 July 2010 content
* rubB (Kanamycin) * ALDH (Kanamycin)

5 KB (699 words) - 19:23, 2 August 2010
Team:BCCS-Bristol/Wetlab/Notebook
...://partsregistry.org/Part:BBa_E0240 BBa_E0240] transformations, and agar + Kanamycin for [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] transformation. L ...g the [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] biobrick are ''Kanamycin'' resistant, not Ampicillin resistant. Will reattempt overnight culture on

17 KB (2,636 words) - 19:06, 16 August 2010
Team:Alberta/Notebook June
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w <ul>14μL either A/B' or B/A' Kanamycin resistance cassette (approx. 100ng/μL)</ul>

37 KB (5,929 words) - 18:34, 4 August 2010
Team:Harvard/vectors/vectors
...nfers plant resistance to <a href="http://en.wikipedia.org/wiki/Kanamycin">kanamycin</a>, to be used in transformant selection.</td> ...r, and pORE R3 the smgfp reporter. Both vectors confer plant resistance to kanamycin. </td>

4 KB (596 words) - 21:37, 24 October 2010
Team:Bielefeld-Germany/Project/Approach
..., a ''vir'' promoter and a readout or selection gene (luciferase, mRFP and kanamycin resistance, respectively): ...stems for the detection of the chosen substances. By varying the amount of kanamycin we will be able to carefully select some promising clones out of thousands

8 KB (1,195 words) - 03:10, 28 October 2010
Team:Calgary/Notebook/Safety And Protocols
...o;L of SOC (30 minutes for Ampicilin resistant plasmids and 60 minutes for kanamycin resistant plasmids) </li> <li> Add Antibiotic (10 µL Ampicillin, 5 µL Kanamycin, or 3 µL Chloramphenicol)</li>

27 KB (4,452 words) - 10:30, 27 October 2010
Team:Freiburg Bioware/testpage
* Kanamycin: 1 g Kanamycin was dissolved in 20 mL multipore-H2O, sterilized by filtration and filled i Further on two control plates containing ampicillin or kanamycin were prepared. Non-transformed cells were plated on them and also stored ov

20 KB (2,960 words) - 12:06, 6 September 2010
Team:Freiburg Bioware/NoteBook/Labjournal/May
* Kanamycin: 1 g Kanamycin was dissolved in 20 mL multipore-H2O, sterilized by filtration and filled i Further on two control plates containing ampicillin or kanamycin were prepared. Non-transformed cells were plated on them and also stored ov

20 KB (2,941 words) - 16:33, 5 January 2011
Team:Freiburg Bioware/NoteBook/Labjournal/August2
...o the standard protocol (XL1B). The cells were plated on a agar plate with Kanamycin. ...Cells: XL1b (60 µL), DNA amount per sample: 3 µL; cells were plated onto Kanamycin-plates.

160 KB (23,468 words) - 21:22, 27 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/September2
2 clones were picked of each ligation approach = 18 falcons (Kanamycin). 2 clones were picked of each ligation approach = 18 falcons (Kanamycin). 

135 KB (19,689 words) - 21:35, 27 October 2010
Team:Brown/Notebook/July21
===Test of kanamycin===

2 KB (324 words) - 23:02, 23 September 2010
Team:UC Davis/notebook/week11.html
...into are already kanamycin resistant. Thus, we need to replace I14015’s kanamycin resistant backbone.</li></ul> 

4 KB (659 words) - 05:50, 27 September 2010
Team:Yale/Our Project/Notebook/Week 7
...tocols/transformation">standard transformation procedure </a> and plate on Kanamycin plates. </li> ...ates, but also transformed DH5alpha cells with Q04121 and plated that with kanamycin. </li>

16 KB (2,556 words) - 02:53, 28 October 2010
Team:Harvard/fences/notebook
*made 4 LB+Kanamycin plates 15μL Kanamycin

74 KB (11,548 words) - 02:19, 27 October 2010
Team:Harvard/results
...nfers plant resistance to <a href="http://en.wikipedia.org/wiki/Kanamycin">kanamycin</a>, to be used in transformant selection.</td> ...r, and pORE R3 the smgfp reporter. Both vectors confer plant resistance to kanamycin. </td>

11 KB (1,766 words) - 18:08, 27 October 2010
Team:Paris Liliane Bettencourt/Project/Memo-cell/Results
...cated in pir+ strains. These plasmids also carried the Chloramphenicol and Kanamycin resistance cassettes. 1% and then replicated on a plate with Kanamycin. Colonies which have excised will

7 KB (1,032 words) - 03:53, 28 October 2010
Team:Calgary/1 August 2010
...ction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today ...of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated.

1 KB (177 words) - 04:54, 23 August 2010