Team:Peking

       Biosensor:
       Based on reverse engineering principles, we
   engineered our bacteria into valid bioreporters
   for Hg(II) in aquatic environment. Rational desi-
   -gn of genetic circuit topology was conducted to
   confer mercury sensor and regulator compone-
   -nts high efficiency and robustness. Additionally,
    a new approach based on the use of multiple
   bioreporter cell lines to control assay variations
   and to improve in field application ease was also
   developed.
                                           ---learn more

       Expansion:    We noticed that MerR family TFs share a high ho-
   -mology at their metal binding domains, which implies that our strategies of    bioreporter and bioabsorbent engineering might be applicable to other cases.
   Then our reverse engineering strategy was expanded to another common to-
   -xic heavy metal, lead (Pb) to prove the validness of our engineering strategy.