Team:Osaka/Notebook

From 2010.igem.org

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(Calendar)
(Notebook)
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==Notebook==
==Notebook==
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===October 1 (Fri)===
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# Gel electrophoresis (?)
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# Ligations
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#* 006: 004 as upstream, FcenA as downstream, 1-5A as vector
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#* 007: 005 as upstream, FcenA as downstream, 1-5A as vector
 +
#* 010: 004 as upstream, Fcex as downstream, 1-5A as vector
 +
#* 011: 005 as upstream, Fcex as downstream, 1-5A as vector
 +
#* 036: 032 as upstream, 033 as downstream, 1-5A as vector
 +
#* 037: 004 as upstream, 024 as downstream, 1-1C as vector
 +
#* 038: 005 as upstream, 024 as downstream, 1-1C as vector
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# Transformation of ligation products
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# PCR of ENO2 promoter, ADH2 promoter, SUC2 signal sequence
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# Parts from NYU team:
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{|
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!ID!!Part Name!!Resistance!!Description
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|-
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|R1||<bbpart>BBa_K416003</bbpart>||A,K||yeast secretion tag
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|-
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|R2||<bbpart>BBa_K416004</bbpart>||A||Aga2 yeast cell surface display tag with linker
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|}
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# Miniprep of 026, 028, 030
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# Restriction digest
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#* 026, 028 with XbaI, PstI
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#* 030 with EcoRI, SpeI or EcoRI only
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# Gel electrophoresis
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# (RESULTS?)
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# Transfer to solution culture: 021, 025, 026, 034, 036
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===TO CHECK/CONFIRM===
===TO CHECK/CONFIRM===
* 'K1' (mentioned on 9/250 -> where did it come from?
* 'K1' (mentioned on 9/250 -> where did it come from?

Revision as of 13:17, 13 October 2010

Calendar

July
Week S M T W T F S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
week1 25 26 27 28 29 30 31
August
Week S M T W T F S
week2 1 2 3 4 5 6 7
week3 8 9 10 11 12 13 14
week4 15 16 17 18 19 20 21
week5 22 23 24 25 26 27 28
week6 29 30 31
September
Week S M T W T F S
week6 1 2 3 4
week7 5 6 7 8 9 10 11
week8 12 13 14 15 16 17 18
week9 19 20 21 22 23 24 25
week10 26 27 28 29 30
October
Week S M T W T F S
week10 1 2
week11 3 4 5 6 7 8 9
week12 10 11 12 13 14 15 16
week13 17 18 19 20 21 22 23
week14 24 25 26 27 28 29 30
week15 31
November
Week S M T W T F S
week15 1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30











Notebook

October 1 (Fri)

  1. Gel electrophoresis (?)
  2. Ligations
    • 006: 004 as upstream, FcenA as downstream, 1-5A as vector
    • 007: 005 as upstream, FcenA as downstream, 1-5A as vector
    • 010: 004 as upstream, Fcex as downstream, 1-5A as vector
    • 011: 005 as upstream, Fcex as downstream, 1-5A as vector
    • 036: 032 as upstream, 033 as downstream, 1-5A as vector
    • 037: 004 as upstream, 024 as downstream, 1-1C as vector
    • 038: 005 as upstream, 024 as downstream, 1-1C as vector
  3. Transformation of ligation products
  4. PCR of ENO2 promoter, ADH2 promoter, SUC2 signal sequence
  5. Parts from NYU team:
IDPart NameResistanceDescription
R1<bbpart>BBa_K416003</bbpart>A,Kyeast secretion tag
R2<bbpart>BBa_K416004</bbpart>AAga2 yeast cell surface display tag with linker
  1. Miniprep of 026, 028, 030
  2. Restriction digest
    • 026, 028 with XbaI, PstI
    • 030 with EcoRI, SpeI or EcoRI only
  3. Gel electrophoresis
  4. (RESULTS?)
  5. Transfer to solution culture: 021, 025, 026, 034, 036



TO CHECK/CONFIRM

  • 'K1' (mentioned on 9/250 -> where did it come from?
  • 9/25 - miniprepped parts (inoculated 9/24) were plasmids from karita sensei? biobrick parts pcr-cloned on 9/23? why where these parts transformed immediately after miniprep?
  • 'CelB, Cel44A' etc vs 'Cel5, Cel8, Cel44' which nomenclature refers to what (received plasmids, pcr product?)