Team:Osaka/week14

From 2010.igem.org

October 24 (Sun)

  1. Transformation
    • 10/23 ligation sample
  2. miniprep
    • 089Y, 100, 131Y, 039Y, 034, 025, 138, 129
  3. Treatment of restriction enzyme
    • 129, 100, 089Y, 131Y, 138, 039
  4. M9 culture
  5. Electrophoresis
    • 039 x
    • 089Y OK
    • 138 x
    • 100 x
    • 129 x
    • 131Y ok
  6. tranformation
    • 91, 93
  7. make amorphous cellulose


October 25 (Mon)

  1. miniprep
    • 039, 107, 129, 138
  2. make Pi buffer
  3. amorphous cellulose
  4. Electrophoresis
    • 138 x
    • 129 ok
    • 107 ok
    • 039 x
  5. transformation
    • 100
  6. To liquid culture
    • 100, 132, 132Y, 089Y, 064-2, 101, 129, 035-2, 094, 123, 126, 132, 091-2, 130


October 26 (Tue)

  1. miniprep
    • 132, 130, 091-2, 123, 126, 091-1, 035-2, 093-1, 013, 129, 100, 089Y, 131, 064-2, 094, 132-1, 132Y, 100
  2. Treatment of restriction enzyme
    • today's miniprep samples
  3. Electrophoresis
    • 013 ok
    • 035 x
    • 089Y ok
    • 091 ok
    • 093 ok
    • 100 x
    • 101 x
    • 126 x
    • 123 x
    • 129 ok
    • 130 ok
    • 132Y ?
    • 064-5  ?
    • 093 ok
    • 094 ok
    • 132 ok
    • 132Y ok
  4. Ligation
    • 039 + 079
    • 075 + 1-23L
    • 2-4A + 102
    • 130 + BYP
  5. measurement OD

October 27 (Wed)

  1. miniprep
  2. Treatment of restriction enzyme
    • 031, 100X, 100Y, 100Z
  3. Electrophoresis
  4. measurement cellulase


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