Team:HokkaidoU Japan/NotebookTest

From 2010.igem.org

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(Monday, August 23, 2010)
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===[[Team:HokkaidoU_Japan/Notebook/August24|Tuesday, August 24, 2010]]===
===[[Team:HokkaidoU_Japan/Notebook/August24|Tuesday, August 24, 2010]]===
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* Electrophoresis of PCR products that had been amplified by using new primers
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* Electrophoresis of PCR products that had been amplified using new primers
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<pre style="color:red">amplified <s>by</s> using</pre>
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* Examination of two ligation kits
* Examination of two ligation kits
* Electrophoresis assay of miscellaneous DNA solutions
* Electrophoresis assay of miscellaneous DNA solutions
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* Transformation with 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium
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* Transformation of 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium
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<pre style="color:red">Transformation of 1-3A</pre>
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===[[Team:HokkaidoU_Japan/Notebook/August25|Wednesday, August 25, 2010]]===
===[[Team:HokkaidoU_Japan/Notebook/August25|Wednesday, August 25, 2010]]===

Revision as of 05:14, 11 September 2010


Calendar

July
SUN MON TUE WED THU FRI SAT
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
SUN MON TUE WED THU FRI SAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
SUN MON TUE WED THU FRI SAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
SUN MON TUE WED THU FRI SAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
November
SUN MON TUE WED THU FRI SAT
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
 

Abstract

Monday, July 12, 2010

  • Our first experiment, transformation of BioBrick devices.

Wednesday, July 21, 2010

  • Preparation of competent cells
  • Single colony isolation of E. coli which had been transformed on Monday, July 12, 2010

Monday, July 26, 2010

  • Cultivation of transformed E. coli for the next day's miniprep

Tuesday, July 27, 2010

  • Miniprep

Monday, August 2, 2010

  • Restriction enzyme digestion of plasmids which had been purified by miniprep
  • Electrophoresis assay

Tuesday, August 3, 2010

Wednesday, August 4, 2010

Thursday, August 5, 2010

Friday, August 6, 2010


Monday, August 9, 2010

  • Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive E. coli and heat-sensitive E. coli
  • These are preliminary experiments before starting the main project

Tuesday, August 10, 2010

  • PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis to confirm

Wednesday, August 11, 2010

  • Preparation for the next day's miniprep
  • Estimation of enzyme activity
  • Preparation of the glycerol stocks

Thursday, August 12, 2010

  • Miniprep (1-18F, 2-21H and 2-11P)
  • Electrophoresis of the DNA which had been amplified via PCR and miniprep

Friday, August 13, 2010

  • PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)

Monday, August 16, 2010

  • Measurement of restriction enzyme activity
  • Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)

Tuesday, August 17, 2010

  • Purification of the DNA solutions via gel extraction
  • Preparation of agar medium containing 35 ug/mL chloramphenicol

Wednesday, August 18, 2010

  • Digestion and gel extraction of 1-2M (retry)
  • 3 piece ligation of 1-18F, 1-23L and pSB1C3
  • Transformation

Thursday, August 19, 2010

  • 3 piece ligation of 3-1E (heat sensor), 1-2M (ribosome binding site) and pSB1C3 (vector)
  • Ligation that uses only vector pSB1C3 to estimate its efficiency

Friday, August 20, 2010

  • PCR amplification of pSB1A3, pSB1C3 and pSB1T3

Monday, August 23, 2010

  • PCR amplification of pSB1A3, pSB1C3 and pSB1T3 (with some changes in protocols)
  • PCR amplification of BioBrick parts using new primers

Tuesday, August 24, 2010

  • Electrophoresis of PCR products that had been amplified using new primers
  • Examination of two ligation kits
  • Electrophoresis assay of miscellaneous DNA solutions
  • Transformation of 1-3A (RFP reporter with chloramphenicol resistance) to evaluate the medium

Wednesday, August 25, 2010

  • Estimation of the amount of 1-3A DNA that could transform and grow cells on the chloramphenicol medium
  • Ethanol precipitation to condense the pSB1C3 DNA solution, and its digestion and ligation
Ligationはお互いそれともパーツと?
  • Digestion of PCR products that had been amplified by using new primers

Thursday, August 26, 2010

  • Electriphoresis to check whether ligation had successed
Electriphoresis to check whether ligation had been successful
  • Digestion of PCR products that had been amplified by using new primers (with reconstruction of reaction system)
amplified by using 

Friday, August 27, 2010

  • Ligation of 1-1A (RFP reporter device) into pSB1C3 and its transformation
  • Retry of 3 piece ligation on August 19th and 20th
Retry of 3 piece ligation which was done on August 19th and 20th
  • Ligation of vector-on-vector
Ligation of vectors to each other

Monday, August 30, 2010

  • Measurement of restriction enzyme activity using highly purified pUC119
  • Digestion of PCR products that had been amplified by using new primers
amplified using

Tuesday, August 31, 2010

  • Gel extraction, ligation and transformation of pUC119

Wednesday, September 1, 2010

  • PCR amplification of 1-5A (RFP reporter)

Thursday, September 2, 2010

  • Digestion, ligation and Transformation of RFP reporter with pSB1C3 or pUC119

Friday, September 3, 2010

  • Colony PCR of previous day's transformed E. coli
Colony PCR of ''E. coli''  transformed on previous day
  • PCR amplification of 1-3A (RFP reporter)

Saturday, September 4, 2010

  • PCR amplification of 1-5A (RFP reporter)
  • Estimation of the amount of 1-3A PCR product

Monday, September 6, 2010

Tuesday, September 7, 2010

Wednesday, September 8, 2010

Thursday, September 9, 2010

Friday, September 10, 2010


Monday, September 13, 2010

Tuesday, September 14, 2010

Wednesday, September 15, 2010

Thursday, September 16, 2010

Friday, September 17, 2010


Monday, September 20, 2010

Tuesday, September 21, 2010

Wednesday, September 22, 2010

Thursday, September 23, 2010

Friday, September 24, 2010


Monday, September 27, 2010

Tuesday, September 28, 2010

Wednesday, September 29, 2010

Thursday, September 30, 2010

Friday, October 1, 2010


Monday, October 4, 2010

Tuesday, October 5, 2010

Wednesday, October 6, 2010

Thursday, October 7, 2010

Friday, October 8, 2010


Monday, October 11, 2010

Tuesday, October 12, 2010

Wednesday, October 13, 2010

Thursday, October 14, 2010

Friday, October 15, 2010


Monday, October 18, 2010

Tuesday, October 19, 2010

Wednesday, October 20, 2010

Thursday, October 21, 2010

Friday, October 22, 2010


Monday, October 25, 2010

Tuesday, October 26, 2010

Wednesday, October 27, 2010

Thursday, October 28, 2010

Friday, October 29, 2010


Monday, November 1, 2010

Tuesday, November 2, 2010

Wednesday, November 3, 2010

Thursday, November 4, 2010

Friday, November 5, 2010


Monday, November 8, 2010

Tuesday, November 9, 2010

Wednesday, November 10, 2010

Thursday, November 11, 2010

Friday, November 12, 2010


Monday, November 15, 2010

Tuesday, November 16, 2010

Wednesday, November 17, 2010

Thursday, November 18, 2010

Friday, November 19, 2010


Monday, November 22, 2010

Tuesday, November 23, 2010

Wednesday, November 24, 2010

Thursday, November 25, 2010

Friday, November 26, 2010


Monday, November 29, 2010

===Tuesday, November 30, 2010===