The degenerate sequences, in which the nucleotide sequences were changed while keeping the primary reading frame of the sequence the same, were all properly screened as SAT sequences. Of the 20 sequences the amino acids, which remained the same as the original sequences, all were identified as SAT sequences while the nucleotides, which were all changed from the original sequences, were undetected as SAT sequences.
This shows the importance of screening the amino-acid too. The algorithm is so robust for codon optimization.

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