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Team:Brown/Notebook/July29
From 2010.igem.org
Thursday, July 29, 2010
Minipreps
Miniprepped overnight cultures using Qiagen kit:
- CI miniprep - 321.8ng/ul
- RBS34 miniprep - 290.8ng/ul
- pLac-Mnt miniprep - 34.7ng/ul
Ligation of WillRS-pNoTat
Successful! Will do an overnight culture tonight and miniprep tomorrow.
Double digests of RBS34 (Bba_B0034), CI
RBS34
50ul total volume
- 5ul NEB 10x buffer 2
- 38ul dH2O
- 1ul EcoRI
- 1ul SpeI
- 5ul miniprep of RBS34
CI
50ul total volume
- 5ul NEB 10x buffer 2
- 38ul dH2O
- 1ul EcoRI
- 1ul Xba1=I
- 5ul miniprep of CI
Gel of double digests
100V for 25 mins on a 1% agarose gel. Lanes:
- Ladder (1kb+)
- CI
- CI
- RBS34
- RBS34
