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Team:Bielefeld-Germany/Project/Protocols
==Determination of minimal inhibitory concentration (MIC) of kanamycin== ...389212 (native ''virA'') in bacteria which are already containing K389101 (kanamycin resistance read out) using [[#Transformation via electroporation |electropo

30 KB (4,241 words) - 02:20, 28 October 2010
Team:Bielefeld-Germany/Project/Theory
...ts. Thus, we wanted to construct our system to lead in the expression of a kanamycin resistance after the induction of the VirA receptor, enabling the quick exc ... expression of aminoglycoside phosphotransferase (APH) that can inactivate kanamycin. The mode of inactivation is the transfer of the y-phosphate from ATP to th

35 KB (5,106 words) - 22:39, 27 October 2010
Team:Alberta/Notebook/BasePlasmids
Our original base plasmids contain a Kanamycin resistance cassette (p1003) bracketed by BsaI or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky

8 KB (1,183 words) - 01:09, 28 October 2010
Team:Alberta/Building Parts
...or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky ends characteristic of an A or a B BioByte. May 10, 2010 PCRed p1003 (Kanamycin cassette)with primers PrA_p1003+ and PrB'_p1003-

30 KB (4,641 words) - 19:55, 19 August 2010
Team:Harvard/vectors/parts
...d>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb" ...d>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.g

8 KB (1,499 words) - 23:19, 26 October 2010
Team:ULB-Brussels/Homologous
...sequences (fig.2). There are two resistance cassette plasmids, one with a kanamycin resistance marker (RCPKan) and the other with a chloramphenicol resistance <leg>Figure 2 : Resistance cassette kanamycin</leg>

19 KB (2,943 words) - 20:44, 27 October 2010
Team:Kyoto/Notebook1
... to LB plate (Amp+). And We didn't pre-culture B0015 despite its vector is Kanamycin-resistance. So, it was predicted that we will fail the transformation of pS |rowspan="2"|1||Kanamycin||○||rowspan="14"|At 37℃, 08/10 20:00-08/11 9:00

55 KB (7,098 words) - 02:17, 28 October 2010
Team:Bielefeld-Germany/Results/Characterization/K389011
...each plate were divided by the total number of colonies on a plate without kanamycin to calculate the survival rate φ. The ability of the bacterial populati ...ced population) indicate a common tendency. Under rising concentrations of kanamycin (x-axis) the survival rate of each population (y-axis) first decreases slow

4 KB (533 words) - 00:59, 28 October 2010
Team:LMU-Munich/Notebook/Apoptosis
- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin) - 3 ml LB-Media + 4 µl Kanamycin

92 KB (13,163 words) - 18:04, 27 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System
...B (Fig3-0-3), production of LuxI is repressed by LasR/3OC12HSL complex and kanamycin resistance gene is activated by LasR/3OC12HSL complex. This means that 3OC1 ...ntration of 3OC12HSL and 3OC6HSL is too low to activate the expressions of kanamycin resistance gene and chloramphenicol resistance gene, respectively. 

13 KB (2,086 words) - 03:59, 28 October 2010
2010.igem.org/Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,317 words) - 18:23, 22 July 2010
Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,354 words) - 03:02, 25 October 2010
Team:Bielefeld-Germany/Results/Used
|style="border-style: solid; border-width: 1px"| Kanamycin resistance gene, <partinfo>K389005</partinfo>: <partinfo>K389005 SpecifiedC ...pectively. It was isolated from the BioBrick <partinfo>P1003</partinfo> (a kanamycin resistance gene with promoter and RBS).

31 KB (4,237 words) - 21:36, 27 October 2010
Team:INSA-Lyon/Project/Stage2/Results
<li>double transformation with ampicillin and kanamycin</li> <li>The lipid part with kanamycin</li>

4 KB (625 words) - 00:36, 28 October 2010
Page79
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the

24 KB (3,715 words) - 22:22, 8 July 2010
Team:Alberta/Notebook May
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR Kanamycin resistance cassette fragment from p1003 containing either the A/B' ends or

31 KB (4,965 words) - 18:29, 4 August 2010
Team:Stanford/Notebook/Lab Work/Week 2
*Transferred 50uL of transformed cells to agar plate with kanamycin. ====Making Kanamycin, Ampicillin Chloramphenicol Plates====

11 KB (1,622 words) - 02:05, 14 September 2010
Team:Bielefeld-Germany/Results/Submitted
===Neomycin / kanamycin resistance=== ...orm. We will use the BioBrick <partinfo>P1003</partinfo> as source for the kanamycin resistance gene.

9 KB (1,365 words) - 02:07, 28 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/September
Next steps: Picking clones of the trafo plates (containing Kanamycin) and perform the Mini-Prep. The obtaining constructs finally will be fused ...rmed according to standard protocol using BL21 cells and plates containing Kanamycin.

196 KB (29,094 words) - 21:34, 27 October 2010
Team:Michigan/Oil Sands August September
''' Transformation of Flu Operon + Kanamycin Backbone Ligation''' ...incubate longer as the slow growth may be due to the high concentration of Kanamycin (50mg/mL) added to the plates.

34 KB (5,403 words) - 01:36, 27 October 2010