Team:Warsaw/Calendar-Stage1/6 July 2010

From 2010.igem.org

(Difference between revisions)
Line 15: Line 15:
<br>2. Alkaline lysis of part of liquid culture used to prepare stocks. Undigested plasmids run on the gel. Results seem to be correct.
<br>2. Alkaline lysis of part of liquid culture used to prepare stocks. Undigested plasmids run on the gel. Results seem to be correct.
 +
<div class="note">Cloning of GFP-terminator behind RBSes - approach 2 </div>
-
3. trawienie wektora z GFP:
+
<br>1. Digest GFP-terminator in psb1A2 plazmid. [16 μl DNA, 6 μl buforu, 16 μl H2O, 0,5 μl Xba/ Pst]
-
16 μl DNA
+
<br>2. Gel extraction of Gel-out GFP-terminator.
-
6 μl buforu
+
<br>3. Ligation of GFPterminator with B0030, B0031, B0032, B0033, B0034 [community RBSes] [1 μl vector-RBSes, 10 μl < insert-GFPterminator, 2 μl buffer, 7 μl water]
-
16 μl H2O
+
<br>4. Transformation with 20 μl ligation mixture
-
0,5 μl Xba/ Pst
+
-
 
+
-
4. Gel-out wstawki GFP
+
-
 
+
-
5. Ligacja GFP z B0030, B0031, B0032, B0033, B0034
+
-
1 μl wektora
+
-
10 μl GFP
+
-
2 μl bufora
+
-
7 μl woda
+
-
 
+
-
 
+
-
6. Transformacja 20 μl mieszaniny ligacyjnej
+
-
7. Wynik transformacji z 05.07.2010: wyrosły podrosty.
+
 +
<div class="note">Cloning of GFP-terminator behind RBSes - approach 1 </div>
 +
7. 05.07.2010 transformation results: contamination with ampicillin susceptible bacteria.
 +
8. Set up one liquiquid culture - mix of clones obtained on the plate.

Revision as of 22:29, 9 July 2010

Example Tabs

Preparation of glycerol stocks

1.Glycerol stocks of folloeing biobricks: J61100, J61101, J61107, J61117, J61127, [Anderson's RBSes] B0030, B0031, B0032, B0033, B0034,[community RBSes] J23100 [synthetic promoter].
2. Alkaline lysis of part of liquid culture used to prepare stocks. Undigested plasmids run on the gel. Results seem to be correct.
Cloning of GFP-terminator behind RBSes - approach 2

1. Digest GFP-terminator in psb1A2 plazmid. [16 μl DNA, 6 μl buforu, 16 μl H2O, 0,5 μl Xba/ Pst]
2. Gel extraction of Gel-out GFP-terminator.
3. Ligation of GFPterminator with B0030, B0031, B0032, B0033, B0034 [community RBSes] [1 μl vector-RBSes, 10 μl < insert-GFPterminator, 2 μl buffer, 7 μl water]
4. Transformation with 20 μl ligation mixture
Cloning of GFP-terminator behind RBSes - approach 1
7. 05.07.2010 transformation results: contamination with ampicillin susceptible bacteria. 8. Set up one liquiquid culture - mix of clones obtained on the plate.

Retrieved from "http://2010.igem.org/Team:Warsaw/Calendar-Stage1/6_July_2010"