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Team:Warsaw/Calendar-Stage1/6 July 2010
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| + | <div class="note"> Preparation of glycerol stocks</div> | ||
| + | <br>1.Glycerol stocks of folloeing biobricks: J61100, J61101, J61107, J61117, J61127, [Anderson's RBSes] B0030, B0031, B0032, B0033, B0034,[community RBSes] J23100 [synthetic promoter]. | ||
| + | <br>2. Alkaline lysis of part of liquid culture used to prepare stocks. Undigested plasmids run on the gel. Results seem to be correct. | ||
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3. trawienie wektora z GFP: | 3. trawienie wektora z GFP: | ||
16 μl DNA | 16 μl DNA | ||
Revision as of 22:19, 9 July 2010
Preparation of glycerol stocks
1.Glycerol stocks of folloeing biobricks: J61100, J61101, J61107, J61117, J61127, [Anderson's RBSes] B0030, B0031, B0032, B0033, B0034,[community RBSes] J23100 [synthetic promoter].
2. Alkaline lysis of part of liquid culture used to prepare stocks. Undigested plasmids run on the gel. Results seem to be correct. 3. trawienie wektora z GFP: 16 μl DNA 6 μl buforu 16 μl H2O 0,5 μl Xba/ Pst 4. Gel-out wstawki GFP 5. Ligacja GFP z B0030, B0031, B0032, B0033, B0034 1 μl wektora 10 μl GFP 2 μl bufora 7 μl woda 6. Transformacja 20 μl mieszaniny ligacyjnej 7. Wynik transformacji z 05.07.2010: wyrosły podrosty.
