Team:Warsaw/Calendar-Stage1/5 July 2010

From 2010.igem.org

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<div class="note"> Confirmation of biobricks obtained from Distribution [Kasia, Jarek]</div>
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1.Finish alkaline lysis no3.
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2.Run 5ul of undigested plasmids on the agarose gel.
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[tu powinien byc zalacznik w postaci obrazka]
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Correct amount of plasmids, expected size.
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<div class="note"> Cloning of GFP-terminator behind RBSes - approach 1</div>
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temat: potwierdzenie ozywienia dobrych brikow
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[Ania1, Ania2, Ania3, Jarek; Kasia]
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1. konczenie minilizy nr3. (Kasia), zel z 5ul nietrawionego plazmidu
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1. Rescue samples from alkaline lysis no.2 by adding RNAse.
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(Jarek)[tu powinien byc zalacznik w postaci obrazka]
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2. SpeI/PstI digest of samples 2-11 (RBSes:). time:3h
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3. XbaI/PstI digest of sample 1 (GFP+terminator). time:3h
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temat: klonowanie GFP-terminator za RBSY (podejscie 1)
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4. Run GFP+terminator on agarose gel [tu powinien byc zalacznik w postaci obrazka] Small amount of plasmid.
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2.(Ania1, Ania2, Ania3, Jarek; Kasia)
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5. Gel extraction of GFP+terminator.
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-Odratowanie minilizy nr 1 przez dodanie RNAzy;
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6. Thermal inactivation of samples 2-6 (Anderson's RBSes) after SpeI/PstI digest.
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-trawienia probek 2-11- RBSY Spe/Pst;
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7. Ligation of digested samples 2-6 (Anderson's RBSes) with gel-extracted GFP-terminator
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-trawienie probki 1-GFP+terminator Xba/Pst; (3godziny)
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8. Transformation with the ligation mixtures.
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-zel GFPterminator [tu powinien byc zalacznik w postaci obrazka] Zel
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wyszedl kiepsko.
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- Gel-out GFPterminator
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-Ligacja trawien probek 2-6 po inaktywacji termicznej z gel-outem GFPterminator
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- Transformacja
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temat: klonowanie GFP-terminator za RBSY (podejscie 2)
temat: klonowanie GFP-terminator za RBSY (podejscie 2)

Revision as of 21:52, 9 July 2010

Example Tabs

Confirmation of biobricks obtained from Distribution [Kasia, Jarek]
1.Finish alkaline lysis no3. 2.Run 5ul of undigested plasmids on the agarose gel. [tu powinien byc zalacznik w postaci obrazka] Correct amount of plasmids, expected size.
Cloning of GFP-terminator behind RBSes - approach 1
[Ania1, Ania2, Ania3, Jarek; Kasia] 1. Rescue samples from alkaline lysis no.2 by adding RNAse. 2. SpeI/PstI digest of samples 2-11 (RBSes:). time:3h 3. XbaI/PstI digest of sample 1 (GFP+terminator). time:3h 4. Run GFP+terminator on agarose gel [tu powinien byc zalacznik w postaci obrazka] Small amount of plasmid. 5. Gel extraction of GFP+terminator. 6. Thermal inactivation of samples 2-6 (Anderson's RBSes) after SpeI/PstI digest. 7. Ligation of digested samples 2-6 (Anderson's RBSes) with gel-extracted GFP-terminator 8. Transformation with the ligation mixtures. temat: klonowanie GFP-terminator za RBSY (podejscie 2) 3. Nastawienie nocnego trawienia GFPterminator 16ul DNA z minilizy 3 w 40 ul. temat: stoki glizerolowe Brikow 4. zasianie brikow do plynu

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