Team:Warsaw/Calendar-Stage1/30 August 2010
From 2010.igem.org
(Difference between revisions)
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<br><div class="note"> Previous day: Set up liquid cultures of E.coli with GFP with different RBS [Ania P.]</div> | <br><div class="note"> Previous day: Set up liquid cultures of E.coli with GFP with different RBS [Ania P.]</div> | ||
- | <br> | + | <br> We used a protocol described <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">here</a> |
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<br><div class="note"> Testing the strenght of RBS [Ania P.,Cherry]</div> | <br><div class="note"> Testing the strenght of RBS [Ania P.,Cherry]</div> | ||
- | <br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer | + | <br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">protocol</a>) |
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<br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div> | <br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div> | ||
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<br /><strong>legend:</strong> | <br /><strong>legend:</strong> | ||
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- | <br />30-08-2010.001- | + | <br />30-08-2010.001- TOP10 without construct |
<br />30-08-2010.007- <a href="http://partsregistry.org/Part:BBa_B0030">B0030</a> | <br />30-08-2010.007- <a href="http://partsregistry.org/Part:BBa_B0030">B0030</a> | ||
<br />30-08-2010.008- <a href="http://partsregistry.org/Part:BBa_B0031">B0031</a> | <br />30-08-2010.008- <a href="http://partsregistry.org/Part:BBa_B0031">B0031</a> |
Revision as of 19:37, 27 October 2010
30.08.2010 Monday
Previous day: Set up liquid cultures of E.coli with GFP with different RBS [Ania P.]
We used a protocol described here
Testing the strenght of RBS [Ania P.,Cherry]
Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol)
Data analysis with Cell Quest and Microsoft Excel[Cherry]
legend:
30-08-2010.001- TOP10 without construct
30-08-2010.007- B0030
30-08-2010.008- B0031
30-08-2010.009- B0032
30-08-2010.010- B0033
30-08-2010.011- B0034
30-08-2010.012- J61100
30-08-2010.013- J61101
30-08-2010.014- J61107
30-08-2010.015- J61117
30-08-2010.016- J61127
Cytometer data processed with Cell Quest:
Cell Quest data processed with Microsoft Excel:
geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events
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