Team:Warsaw/Calendar-Stage1/17 August 2010
From 2010.igem.org
17.08.2010 Tuesday
Previous day: Set up liquid cultures of E.coli TOP10 with measured comstructs [Ania O.]
We used a protocol described here
Testing the strenght of RBS [Ania O.,Cherry]
Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol
Data analysis with Cell Quest and Microsoft Excel[Cherry]
Legend:
17-08-2010.001- TOP10
17-08-2010.015- J61100
17-08-2010.016- J61101
17-08-2010.017- J61107
17-08-2010.018- J61117
17-08-2010.019- J61127
17-08-2010.020- B0030
17-08-2010.021- B0031
17-08-2010.022- B0032
17-08-2010.023- B0033
17-08-2010.024- B0034
For pdf version click here.
Cytometer data processed with Cell Quest:
Cell Quest data processed with Microsoft Excel:
geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events
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