Team:Valencia/Project

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The implementation of the project has several parts and sub-objectives.

Martian conditions simulation chamber

We want to reproduce some of the characteristics that make difficult the life in Mars. Among them we can have the air pressure between 7 and 10 mbar, the gases composition, the high temperature range going from -70ºC to 20ºC in some parts of the planet. Once we achieve this humble goal, we can use the chamber to try our engineered yeasts with the LEA gene and observe its behavior: if it grows, just only survives without growing or dies. It’s a very important part of the project because is an easy way to prove whether the yeast can grow or at least survive in an environment with such limitations as Martian atmosphere can be.

Red-house

In the other hand, at some situation in Mars terraformation process, our microorganisms are going to need an appropriate (or cozy) environment to grow. Regarding this, we are going to build a “Red-House” (an analogy with a greenhouse, but in Mars!!) in order to preserve the growing cultures until they are ready to go to live in somewhere else. The Red-house will be a device designed to protect the microorganisms from the harsh conditions of: temperature, pressure and radiation. Once the device is thermally isolated from the exterior media, we are trying to warm its interior with electricity generated using wind turbines.

Prionic Switch test

The third phase is the test of the prionic switch in the original configuration. We want to test how the prionic switch respond to heat shock and another stress inputs, and if it inhibits the expression of the betaGAL reporter. In this situation we also want to see the effect of “the color” of the cultures in its surface temperature. Hopefully betaGAL blue is better (warmer) than white and red (Martian red).

LEA Experiments in E. Coli

The fourth part involves dealing with violent temperature changes of the Martian surface. To do this we will implement the expression of LEA (late embryogenesis abundant) “antifreeze” protein. Thus, we want to verify the resistance to cold shock and salt stress of the E. coli cultures who which express LEA and compare this result with the ones who doesn't (control cultures). This brings the new problem of which control cultures we shall use. The same E.coli with the pM2 plasmid but without the insert (this is probably the best option but the more difficult one) What happened with this issue guys???

Expresing LEA in Yeasts

The fifth part is to express LEA in Saccharomices Cereviciae. We have the W303 strain. We need to put together the LEA gene in an expression vector for Eukaryotes with a constitutive promoter and probably with a HSP promoter. (Update on this topic, please)

Melanin sinthesis modulation in Yeasts with a temperature activated prionic switch

Explanation needed here (Jose maybe???)