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Team:UT-Tokyo/Sudoku experiment
From 2010.igem.org
Sudoku
Abstract Construct Lab note Result
Lab note
This is an abstract page. If you can see detail data, please click each protocols. This is an abstract page. If you can see detail data, please click each protocols.
Pre-experiment
Make construct
Detail protocols about this is:
Check test
Check test
| Number | Name | Link to BioBrick | Plate coordinate | Vector | Code length |
| 1 | T7 promoter | plate1-6N | pSB1AK8 | 46bp | |
| 2 | rbs | plate1-1H | pSB1A2 | 15bp | |
| 3 | cre recombinase | plate1-5D | pSB1A2 | 1037bp | |
| 4 | double terminator | plate2-24C | pSB1AK3 | 95bp | |
| 5 | lox66 recombinase site | plate1-17J | pSB1A2 | 34bp | |
| 6 | Kan resistance (rev) | plate1-2K | pSB1A2 | 816bp | |
| 7 | rbs (rev) | plate1-1J | pSB1A2 | 15bp | |
| 8 | lox71 recombinase site |
order-made | --- | --- | 34bp |
| 9 | single terminator | plate1-4H | pSB1AK3 | 39bp | |
| 10 | constant express promoter | plate1-18A | pSB1A2 | 35bp | |
| 11 | Tet resistance (rev) | plate1-1N | pSB1A2 | 1191bp | |
| 12 | hixC | plate1-1B | pSB1A2 | 26bp | |
| 13 | --- |
--- | --- | --- | --- |
| 14 | lox66 | plate1-17H | pSB1A2 | 34bp | |
| 15 | rbs-mRFP1-terminator | plate1-22O | pSB1A2 | 861bp | |
| 16 | location sequence(old) |
order | --- | --- | --- |
| 17 | pSP6(rev) |
order | --- | --- | --- |
| 18 | lox2272 |
order | --- | --- | --- |
| 19 | loading sequence(rev) |
order | --- | --- | --- |
| 20 | frt | From HQ | pSB1A2 | 34bp | |
| 21 | hin(true) | From HQ | ??? | 573bp | |
| 22 | hin(rev) |
--- | --- | ??? | 573bp |
| 23 | flpe |
--- | --- | --- | about 1.2kbp |
| 24 | gfp unit |
--- | --- | --- | about 1.2kbp |
| 25 | flpe(rev) |
--- | --- | --- | about 1.2kbp |
Main construct
Research of SP6 RNApol cDNA and F plasmid
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Make Hin(rev)
Revise MS2 gene
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
Make location(rev)-rbs(rev)-location(rev)
